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UDP-glycosyltransferases contribute to spirotetramat resistance in Aphis gossypii Glover
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.pestbp.2020.104565 Yiou Pan 1 , Shuyuan Wen 2 , Xuewei Chen 3 , Xiwu Gao 4 , Xiaochun Zeng 2 , Xuemei Liu 2 , Fayi Tian 2 , Qingli Shang 1
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.pestbp.2020.104565 Yiou Pan 1 , Shuyuan Wen 2 , Xuewei Chen 3 , Xiwu Gao 4 , Xiaochun Zeng 2 , Xuemei Liu 2 , Fayi Tian 2 , Qingli Shang 1
Affiliation
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Uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyze the conjugation of small lipophilic endogenous and exogenous compounds with sugars to produce water-soluble glycosides, playing an important role in insect endobiotic regulation and xenobiotic detoxification. In this study, two UGT-inhibitors, sulfinpyrazone and 5-nitrouracil, significantly increased spirotetramat toxicity against third instar nymphs of resistant Aphis gossypii, whereas there were no synergistic effects in apterous adult aphids, suggesting UGT involvement in spirotetramat resistance in cotton aphids. Furthermore, the UHPLC-MS/MS was employed to determine the content of spirotetramat and its four metabolites (S-enol, S-glu, S-mono, S-keto) in the honeydew of resistant cotton aphids under spirotetramat treatment. No residual spirotetramat was detected in the honeydew, while its four metabolites were detected at a S-enol: S-glu: S-mono: S-keto ratio of 69.30: 6.54: 1.44: 1.00. Therefore, glycoxidation plays a major role in spirotetramat inactivation and excretion in resistant aphids. Compared with the susceptible strain, the transcriptional levels of UGT344M2 were significantly upregulated in nymphs and adults of the resistant strain. RNA interference of UGT344M2 dramatically increased spirotetramat toxicity in nymphs, but no such effect were found in the resistant adult aphids. Overall, UGT-mediated glycoxidation were found to be involved in spirotetramat resistance. The suppression of UGT344M2 significantly increased the sensitivity of resistant nymphs to spirotetramat, suggesting that UGT344M2 upregulation might be associated with spirotetramat detoxification. This study provides an overview of the involvement of metabolic factors, UGTs, in the development of spirotetramat resistance.
中文翻译：
UDP-糖基转移酶有助于棉蚜的螺虫抗性
尿苷二磷酸 (UDP)-糖基转移酶 (UGTs) 催化小的亲脂性内源性和外源性化合物与糖结合产生水溶性糖苷，在昆虫内生调控和外源性解毒中发挥重要作用。在这项研究中，两种 UGT 抑制剂磺吡酮和 5-硝基尿嘧啶显着增加了螺虫乙酯对抗性棉蚜三龄若虫的毒性，而在无翅成虫中没有协同作用，表明 UGT 参与了棉蚜对螺虫乙酯的抗性。此外，采用UHPLC-MS/MS测定了螺虫乙酯处理下抗性棉蚜蜜露中螺虫乙酯及其四种代谢物（S-烯醇、S-glu、S-mono、S-keto）的含量。在蜜露中未检测到残留的螺虫乙酯，而其四种代谢物的 S-烯醇：S-glu：S-单：S-酮比为 69.30：6.54：1.44：1.00。因此，糖氧化在抗性蚜虫的螺虫乙酯灭活和排泄中起主要作用。与易感菌株相比，UGT344M2的转录水平在抗性菌株的若虫和成虫中显着上调。UGT344M2的RNA干扰显着增加了若虫中螺虫的毒性，但在抗性成虫蚜虫中没有发现这种影响。总体而言，发现 UGT 介导的糖氧化与螺虫乙酯抗性有关。UGT344M2的抑制显着增加了抗性若虫对螺虫乙酯的敏感性，表明UGT344M2上调可能与螺虫乙酯解毒有关。
更新日期：2020-06-01
中文翻译：
UDP-糖基转移酶有助于棉蚜的螺虫抗性
尿苷二磷酸 (UDP)-糖基转移酶 (UGTs) 催化小的亲脂性内源性和外源性化合物与糖结合产生水溶性糖苷，在昆虫内生调控和外源性解毒中发挥重要作用。在这项研究中，两种 UGT 抑制剂磺吡酮和 5-硝基尿嘧啶显着增加了螺虫乙酯对抗性棉蚜三龄若虫的毒性，而在无翅成虫中没有协同作用，表明 UGT 参与了棉蚜对螺虫乙酯的抗性。此外，采用UHPLC-MS/MS测定了螺虫乙酯处理下抗性棉蚜蜜露中螺虫乙酯及其四种代谢物（S-烯醇、S-glu、S-mono、S-keto）的含量。在蜜露中未检测到残留的螺虫乙酯，而其四种代谢物的 S-烯醇：S-glu：S-单：S-酮比为 69.30：6.54：1.44：1.00。因此，糖氧化在抗性蚜虫的螺虫乙酯灭活和排泄中起主要作用。与易感菌株相比，UGT344M2的转录水平在抗性菌株的若虫和成虫中显着上调。UGT344M2的RNA干扰显着增加了若虫中螺虫的毒性，但在抗性成虫蚜虫中没有发现这种影响。总体而言，发现 UGT 介导的糖氧化与螺虫乙酯抗性有关。UGT344M2的抑制显着增加了抗性若虫对螺虫乙酯的敏感性，表明UGT344M2上调可能与螺虫乙酯解毒有关。
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