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Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
Biomaterials Advances ( IF 5.5 ) Pub Date : 2020-03-23 , DOI: 10.1016/j.msec.2020.110897
Sedef İlk 1 , Aurelija Ramanauskaitė 2 , Behlül Koç Bilican 3 , Povilas Mulerčikas 4 , Dilek Çam 5 , M Serdar Onses 6 , Ilker Torun 7 , Sonata Kazlauskaitė 4 , Vykintas Baublys 2 , Ömer Aydın 8 , Lian-Sheng Zang 9 , Murat Kaya 3
Affiliation  

Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature. In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films. Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size. However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability. In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier. A model drug, quercetin, was loaded into chitosan membrane, and its release profile was examined. Also, a point-of-care test was conducted for both chitin and chitosan membranes. Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies. It was observed that chitosan membrane could be used as a drug carrier material. Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.



中文翻译:

从昆虫角膜晶状体获得的天然壳聚糖膜作为药物载体的用途及其在即时检验中的潜力。

壳聚糖具有无毒,可生物降解,生物相容性,抗微生物和抗氧化等特性,是用作药物载体的必不可少的生物聚合物。在以前的研究中,脱乙酰壳多糖首先溶解在弱酸中并形成凝胶,然后用于携带药物活性化合物,例如纳米颗粒,胶囊,复合材料和薄膜。在将其溶于弱酸中之后使用制得的壳聚糖凝胶具有优点,例如易于加工以装载所需量的活性物质并制成所需的形状和大小。但是,溶解的壳聚糖失去了其某些自然特性,例如纤维结构,结晶度和热稳定性。在这项研究中,首次从昆虫的(Tabanus bovinus)将原始形状完整的复眼作为药物载体进行测试。将模型药物槲皮素加载到壳聚糖膜中,并检查其释放曲线。另外,对几丁质和壳聚糖膜均进行了即时检验。通过使用FTIR,TGA,元素分析和表面润湿性分析以及立体,双目和扫描电子显微镜对从昆虫角膜镜片获得的甲壳质和壳聚糖膜进行表征。观察到壳聚糖膜可以用作药物载体材料。壳多糖膜和壳聚糖膜都将得到改进,以用于侧向流动测定,并且可以在进一步研究中对这些膜进行其他生物工程应用的测试。

更新日期:2020-03-23
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