Rapid detection of Acinetobacter baumannii (AB) is critical for limiting healthcare-associated infections and providing the best treatment for infected individuals. Herein an integrated microfluidic device for AB diagnosis utilizing a new dual aptamer assay was developed for point-of-care (POC) applications; magnetic beads coated with AB-specific aptamers were used to capture bacteria, and quantum dots (QD) bound to a second aptamer were utilized to quantify the amount of bacteria with a light-emitting diode (LED)-induced fluorescence module integrated into the device. Within a rapid detection of 30 min, a limit of detection of only 100 colony-forming units (CFU)/reaction was obtained, and all necessary microfluidic devices were actuated by a combination of permanent magnets and electromagnets. The pumping rate of the micropump was 270 μL/min at only 10 V, which is amenable for POC applications with lower power consumption, and only 10 μL of sample and reagents were required. Given these attributes, an automatic POC device was demonstrated which could perform a dual aptamer assay to diagnose AB by using electromagnetically-driven microfluidic system. This system provides a rapid, sensitive, low power and reagents consumption and fully automated for AB detection by using a dual aptamer assay. It will allow rapid clinical diagnosis of AB in the near future.

中文翻译：

---

用于在电磁驱动的微流控平台上检测鲍曼不动杆菌的双重适体测定。

快速检测鲍曼不动杆菌（AB）对于限制与医疗保健相关的感染并为受感染的个体提供最佳治疗至关重要。在本文中，开发了一种利用新型双重适体测定法进行AB诊断的集成微流控设备，用于现场护理（POC）应用。使用涂有AB特异性适体的磁珠捕获细菌，并利用集成到设备中的发光二极管（LED）诱导的荧光模块，利用与第二个适体结合的量子点（QD）定量细菌数量。在30分钟的快速检测中，仅获得了100个菌落形成单位（CFU）/反应的检测极限，并且所有必需的微流体装置均由永磁体和电磁体共同驱动。微型泵的泵速仅在10 V时为270μL/ min，适合功耗较低的POC应用，并且仅需10μL的样品和试剂。考虑到这些属性，演示了一种自动POC设备，该设备可以通过使用电磁驱动的微流体系统执行双重适体测定来诊断AB。该系统提供了快速，灵敏，低功耗和试剂消耗的功能，并且通过使用双适体测定实现了全自动的AB检测。它可以在不久的将来对AB进行快速临床诊断。演示了一种自动POC设备，该设备可以通过使用电磁驱动的微流控系统执行双适体分析来诊断AB。该系统提供了快速，灵敏，低功耗和试剂消耗的功能，并且通过使用双适体测定实现了全自动的AB检测。它可以在不久的将来对AB进行快速临床诊断。演示了一种自动POC设备，该设备可以通过使用电磁驱动的微流控系统执行双适体分析来诊断AB。该系统提供了快速，灵敏，低功耗和试剂消耗的功能，并且通过使用双适体测定实现了全自动的AB检测。它可以在不久的将来对AB进行快速临床诊断。