BACKGROUND This study sought to detect the expression and clinical significance of miR-4516 and miR-21-5p in serum of patients with colorectal cancer. METHODS Bioinformatics methods were used to analyze the expression patterns of miR-4516 and miR-21-5p in colorectal cancer. A total of 80 patients with colorectal cancer, 65 patients with benign colorectal tumors and 50 healthy persons were selected. qRT-PCR was performed to detect the expression levels of serum miR-4516 and miR-21-5p before and after operation or postoperative recurrence. The correlation of miR-4516 and miR-21-5p expression levels with the clinical characteristics and prognosis of colorectal cancer was analyzed, and that with the patient's survival was further examined by Kaplan-Meier analysis. RESULTS MiR-4516 was poorly expressed in colorectal cancer in the preoperative group, and miR-21-5p was highly expressed. While in the postoperative group, miR-4516 was up-regulated, and miR-21-5p was down-regulated. The low expression of miR-4516 was shown to be related to TNM staging, invasion degree, lymph node metastasis and distant metastasis of the patients. Whereas the high expression of miR-21-5p was proved to be correlated with TNM staging and lymph node metastasis. Kaplan-Meier survival analysis showed that high expression of miR-4516 or low expression of miR-21-5p could contribute to better overall survival. CONCLUSION Low miR-4516 or high miR-21-5p could be used as an independent risk factor for prognosis of colorectal cancer.

中文翻译：

---

大肠癌患者血清中miR-4516和miR-21-5p的表达及其临床意义。

背景技术本研究试图检测miR-4516和miR-21-5p在结直肠癌患者血清中的表达及其临床意义。方法采用生物信息学方法分析miR-4516和miR-21-5p在大肠癌中的表达模式。总共选择了80例结直肠癌患者，65例良性结直肠肿瘤患者和50名健康人。术前或术后或术后复发进行qRT-PCR检测血清miR-4516和miR-21-5p的表达水平。分析了miR-4516和miR-21-5p表达水平与大肠癌的临床特征和预后的相关性，并通过Kaplan-Meier分析进一步检查了其与患者生存率的相关性。结果术前组大肠癌中MiR-4516的表达较弱，miR-21-5p的表达较高。在术后组中，miR-4516被上调，而miR-21-5p被下调。结果表明，miR-4516的低表达与患者的TNM分期，侵袭程度，淋巴结转移和远处转移有关。事实证明，miR-21-5p的高表达与TNM分期和淋巴结转移有关。Kaplan-Meier生存分析表明，miR-4516的高表达或miR-21-5p的低表达可能有助于改善总体生存率。结论低miR-4516或高miR-21-5p可以作为结直肠癌预后的独立危险因素。而miR-21-5p被下调。结果表明，miR-4516的低表达与患者的TNM分期，侵袭程度，淋巴结转移和远处转移有关。事实证明，miR-21-5p的高表达与TNM分期和淋巴结转移有关。Kaplan-Meier生存分析表明，miR-4516的高表达或miR-21-5p的低表达可能有助于更好的总体生存。结论低miR-4516或高miR-21-5p可以作为结直肠癌预后的独立危险因素。而miR-21-5p被下调。miR-4516的低表达与患者的TNM分期，浸润程度，淋巴结转移和远处转移有关。事实证明，miR-21-5p的高表达与TNM分期和淋巴结转移有关。Kaplan-Meier生存分析表明，miR-4516的高表达或miR-21-5p的低表达可能有助于更好的总体生存。结论低miR-4516或高miR-21-5p可以作为结直肠癌预后的独立危险因素。Kaplan-Meier生存分析表明，miR-4516的高表达或miR-21-5p的低表达可能有助于改善总体生存率。结论低miR-4516或高miR-21-5p可以作为结直肠癌预后的独立危险因素。Kaplan-Meier生存分析表明，miR-4516的高表达或miR-21-5p的低表达可能有助于改善总体生存率。结论低miR-4516或高miR-21-5p可以作为结直肠癌预后的独立危险因素。