Comprehensive In Vitro Metabolism Study of Bisphenol A Using Liquid Chromatography-High Resolution Tandem Mass Spectrometry.,Chemical Research in Toxicology

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Comprehensive In Vitro Metabolism Study of Bisphenol A Using Liquid Chromatography-High Resolution Tandem Mass Spectrometry.
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2020-03-24 , DOI: 10.1021/acs.chemrestox.0c00042
Ons Ousji ₁ , Leanne Ohlund ₁ , Lekha Sleno ₁

Affiliation

Bisphenol A (BPA) metabolism has been investigated using several in vitro models, including human and rat liver microsomes and subcellular (S9) fractions, as well as human-recombinant cytochrome P450 3A4 (CYP3A4) expressed in Supersomes, for a comprehensive look at all possible metabolic pathways. By an untargeted approach using liquid chromatography coupled to a high-resolution quadrupole-time-of-flight mass spectrometer, we were able to detect a large number of known Phase I and Phase II metabolites of BPA, as well as several previously uncharacterized ones. A detailed fragmentation study of BPA and its detected metabolites was crucial to confirm structures. Isotope-labeled BPA analogs were highly useful for the structural elucidation of many metabolites. These results contribute to a better understanding of BPA metabolism, including pathways that may introduce additional toxicity, as well as help with the assessment of BPA exposure in different biological matrices.

中文翻译：

使用液相色谱-高分辨率串联质谱法对双酚A进行全面的体外代谢研究。

双酚A（BPA）代谢已使用几种体外方法进行了研究模型，包括人和大鼠肝微粒体和亚细胞（S9）组分，以及在Supersomes中表达的人重组细胞色素P450 3A4（CYP3A4），以全面了解所有可能的代谢途径。通过使用液相色谱与高分辨率四极杆飞行时间质谱仪联用的非靶向方法，我们能够检测出大量已知的BPA的I期和II期代谢物，以及一些以前未表征的代谢物。对BPA及其检测到的代谢物进行详细的片段化研究对于确定结构至关重要。同位素标记的BPA类似物对许多代谢物的结构阐明非常有用。这些结果有助于更好地理解BPA代谢，包括可能引入其他毒性的途径，

更新日期：2020-03-24

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