High-Strength Fiber-Reinforced Composite Hydrogel Scaffolds as Biosynthetic Tendon Graft Material,ACS Biomaterials Science & Engineering

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High-Strength Fiber-Reinforced Composite Hydrogel Scaffolds as Biosynthetic Tendon Graft Material
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-03-22 , DOI: 10.1021/acsbiomaterials.9b01716
Young Jung No _{1,

2} , Solaiman Tarafder ₃ , Barbara Reischl ₄ , Yogambha Ramaswamy _{1,

2} , Colin Dunstan _{1,

2} , Oliver Friedrich ₄ , Chang Hun Lee ₃ , Hala Zreiqat _{1,

2}

Affiliation

The development of suitable synthetic scaffolds for use as human tendon grafts to repair tendon ruptures remains a significant engineering challenge. Previous synthetic tendon grafts have demonstrated suboptimal tissue ingrowth and synovitis due to wear particles from fiber-to-fiber abrasion. In this study, we present a novel fiber-reinforced hydrogel (FRH) that mimics the hierarchical structure of the native human tendon for synthetic tendon graft material. Ultrahigh molecular weight polyethylene (UHMWPE) fibers were impregnated with either biosynthetic polyvinyl alcohol/gelatin hydrogel (FRH-PG) or with polyvinyl alcohol/gelatin + strontium-hardystonite (Sr-Ca₂ZnSi₂O₇, Sr-HT) composite hydrogel (FRH-PGS). The scaffolds were fabricated and assessed to evaluate their suitability for tendon graft applications. The microstructure of both FRH-PG and FRH-PGS showed successful impregnation of the hydrogel component, and the tendon scaffolds exhibited equilibrium water content of ∼70 wt %, similar to the values reported for native human tendon, compared to ∼50 wt % water content retained in unmodified UHMWPE fibers. The tensile strength of FRH-PG and FRH-PGS (77.0–81.8 MPa) matched the range of human Achilles’ tendon tensile strengths reported in the literature. In vitro culture of rat tendon stem cells showed cell and tissue infiltration into both FRH-PG and FRH-PGS after 2 weeks, and the presence of Sr-HT ceramic particles influenced the expression of tenogenic markers. On the other hand, FRH-PG supported the proliferation of murine C2C12 myoblasts, whereas FRH-PGS seemingly did not support it under static culture conditions. In vivo implantation of FRH-PG and FRH-PGS scaffolds into full-thickness rat patellar tendon defects showed good collagenous tissue ingrowth into these scaffolds after 6 weeks. This study demonstrates the potential viability for our FRH-PG and FRH-PGS scaffolds to be used for off-the-shelf biosynthetic tendon graft material.

中文翻译：

高强度纤维增强复合水凝胶支架作为生物合成肌腱接枝材料

用作人的肌腱移植物以修复肌腱破裂的合适的合成支架的开发仍然是重大的工程挑战。先前的合成肌腱移植物由于纤维间磨损产生的磨损颗粒，已证明组织向内生长不足和滑膜炎。在这项研究中，我们提出了一种新型的纤维增强水凝胶（FRH），该凝胶模仿了人工合成肌腱移植材料的天然人肌腱的层次结构。用生物合成聚乙烯醇/明胶水凝胶（FRH-PG）或聚乙烯醇/明胶+锶-硬石膏（Sr-Ca ₂ ZnSi ₂ O ₇）浸渍超高分子量聚乙烯（UHMWPE）纤维。（Sr-HT）复合水凝胶（FRH-PGS）。搭建并评估支架以评估其在肌腱移植中的适用性。FRH-PG和FRH-PGS的微观结构均成功浸渍了水凝胶成分，腱支架的平衡水含量约为70重量％，与约50重量％的水相比，与天然人腱的报道值相似。含量保留在未改性的UHMWPE纤维中。FRH-PG和FRH-PGS的拉伸强度（77.0-81.8 MPa）与文献中报道的人类跟腱的拉伸强度范围相匹配。大鼠肌腱干细胞的体外培养显示2周后细胞和组织均浸入FRH-PG和FRH-PGS中，并且Sr-HT陶瓷颗粒的存在影响肌腱标记的表达。另一方面，FRH-PG支持鼠C2C12成肌细胞的增殖，而FRH-PGS在静态培养条件下似乎不支持它。FRH-PG和FRH-PGS支架在体内植入全厚度大鼠pa腱缺损显示6周后，良好的胶原组织向内生长。这项研究证明了我们的FRH-PG和FRH-PGS支架用于现成的生物合成腱移植材料的潜在可行性。

更新日期：2020-04-23

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