Nanofocused Scanning X-ray Fluorescence Microscopy Revealing an Effect of Heterozygous Hemoglobin S and C on Biochemical Activities in Plasmodium falciparum-Infected Erythrocytes.,Analytical Chemistry

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Nanofocused Scanning X-ray Fluorescence Microscopy Revealing an Effect of Heterozygous Hemoglobin S and C on Biochemical Activities in Plasmodium falciparum-Infected Erythrocytes.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-31 , DOI: 10.1021/acs.analchem.9b05111
Benjamin Fröhlich ₁ , Yang Yang _{2,

3} , Judith Thoma ₁ , Julian Czajor ₁ , Christine Lansche ₄ , Cecilia Sanchez ₄ , Michael Lanzer ₄ , Peter Cloetens ₂ , Motomu Tanaka _{1,

5}

Affiliation

While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life-threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spatial distribution of individual elements in subcellular compartments, including Fe, S, P, Zn, and Cu, in Plasmodium falciparum-infected (P. falciparum-infected) erythrocytes carrying the wild type or variant hemoglobins. Our data indicate that heterozygous hemoglobin S and C significantly modulate biochemical reactions in parasitized erythrocytes, such as aberrant hemozoin mineralization and a delay in hemoglobin degradation. The label-free scanning X-ray fluorescence imaging has great potential to quantify the spatial distribution of elements in subcellular compartments of P. falciparum-infected erythrocytes and unravel the biochemical mechanisms underpinning disease and protective traits.

中文翻译：

纳米聚焦扫描X射线荧光显微镜揭示了杂合血红蛋白S和C对恶性疟原虫感染的红细胞生化活性的影响。

尽管有充分的证据表明杂合血红蛋白S和C的携带者受到保护，免于危及生命的疟疾，但对于单细胞水平的潜在生化机制知之甚少。使用纳米聚焦扫描X射线荧光显微镜，我们量化了携带野生型的恶性疟原虫感染（恶性疟原虫感染）红细胞在亚细胞区室中各个元素的空间分布，包括Fe，S，P，Zn和Cu或变体血红蛋白。我们的数据表明，杂合的血红蛋白S和C显着调节了寄生红细胞中的生化反应，例如异常的血红蛋白矿化和血红蛋白降解的延迟。

更新日期：2020-04-23

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