BACKGROUND Human papillomaviruses (HPVs) have been linked to a variety of human cancers. As the landscape of HPV-related neoplasia continues to expand, uncommon and rare HPV genotypes have also started to emerge. Host-virus interplay is recognized as a key driver in HPV carcinogenesis, with host immune status, virus genetic variants and coinfection highly influencing the dynamics of malignant transformation. Immunosuppression and tissue tropism are also known to influence HPV pathogenesis. METHODS Herein, we present a case of a patient who, in the setting of HIV positivity, developed anal squamous cell carcinoma associated with HPV69 and later developed squamous cell carcinoma in the lungs, clinically presumed to be metastatic disease, associated with HPV73. Consensus PCR screening for HPV was performed by real-time PCR amplification of the L1 gene region, amplification of the E6 regions with High-Resolution Melting Curve Analysis followed by Sanger sequencing confirmation and phylogenetic analysis. RESULTS Sanger sequencing of the consensus PCR amplification product determined that the anal tissue sample was positive for HPV 69, and the lung tissue sample was positive for HPV 73. CONCLUSIONS This case underscores the importance of recognizing the emerging role of these rare "possibly carcinogenic" HPV types in human carcinogenesis.

中文翻译：

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人乳头瘤病毒（HPV69 / HPV73）合并感染与肛门同时鳞状上皮癌和推测的肺转移有关。

背景技术人类乳头瘤病毒（HPV）已经与多种人类癌症相关。随着与HPV相关的肿瘤形成的局面不断扩大，不常见和罕见的HPV基因型也开始出现。宿主与病毒的相互作用被认为是HPV致癌作用的关键驱动因素，宿主的免疫状态，病毒遗传变异和共感染极大地影响着恶性转化的过程。还已知免疫抑制和组织嗜性影响HPV的发病机理。方法在本文中，我们介绍了一名患者，该患者在HIV阳性的情况下发展为与HPV69相关的肛门鳞状细胞癌，后来又在临床上被认为是转移性疾病的与HPV73相关的肺部发展为鳞状细胞癌。对HPV的共识性PCR筛选是通过实时PCR扩增L1基因区域，用高分辨率熔解曲线分析扩增E6区域，然后进行Sanger测序确认和系统发育分析来进行的。结果共有PCR扩增产物的Sanger测序确定肛门组织样品中HPV 69阳性，肺组织样品中HPV 73阳性。结论该病例强调了认识这些罕见的“可能致癌”作用的重要性。 HPV在人类致癌中的类型。