The herbal drug incorporated nanofibers with potential accelerating wound healing has become a promising wound dressing to treat wounds. The present research aimed to study the effects of adding different amounts of Elaeagnus Angustifolia (EA) extract on physical, mechanical, and biological properties of silk fibroin-polyvinyl alcohol (SF-PVA) nanofibers. Thus, different electrospun mats were made by changing the SF to the EA ratio. The successful incorporation of EA in SF-PVA nanofibers was confirmed by Fourier transform infrared spectroscopy (FTIR). No obvious change was observed in the mean fiber diameter except for EA/SF ratio 1:3. Also, mechanical properties results showed that the ultimate tensile strength of samples dropped from 9±0.8 MPa to 2.50±0.03 MPa with decreasing SF amount. The in vitro release studies indicated that the fickian diffusion controlled the EA release profile and it was faster in the nanofibers containing more EA. Furthermore, the addition of EA into the nanofibrous dressings improved their antioxidant activity as well as antibacterial activity against both gram-positive (S. aureus) and gram- negative (E. coli) bacteria. Cell viability was also measured using fibroblast cells cultured on prepared dressings and results proved that the higher amounts of EA have a significant impact on cell viability. These findings suggest the advantages of EA as an additive to regulate cell behavior and for skin tissue regeneration.

含有纳米纤维的草药药物具有加速伤口愈合的潜力，已成为治疗伤口的有希望的伤口敷料。本研究旨在研究添加不同量的沙枣（EAaeagnus Angustifolia（EA）提取物）对丝素蛋白-聚乙烯醇（SF-PVA）纳米纤维的物理，机械和生物学特性的影响。因此，通过将SF更改为EA比例来制作不同的电纺垫。EA的成功合并傅立叶变换红外光谱法（FTIR）证实了SF-PVA纳米纤维中的纳米粒子含量。除了EA / SF比为1：3之外，平均纤维直径没有观察到明显变化。另外，力学性能结果表明，随着SF含量的降低，样品的极限抗拉强度从9±0.8 MPa降至2.50±0.03 MPa。体外释放研究表明，菲克扩散控制了EA的释放曲线，并且在包含更多EA的纳米纤维中速度更快。此外，在纳米纤维敷料中添加EA可以提高其抗氧化活性以及对革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌）的抗菌活性。）细菌。还使用在准备好的敷料上培养的成纤维细胞来测量细胞活力，结果证明较高含量的EA对细胞活力具有重大影响。这些发现表明EA作为调节细胞行为和皮肤组织再生的添加剂的优点。