Abstract Chlorpyrifos is an organophosphorus pesticide widely used in agriculture and known to cause environmental pollution, as well as negatively impact human health. In this study, we reported a cytometric bead array (CBA) method to detect chlorpyrifos. Magnetic microspheres (Fe3O4@SiO2 COOH) were prepared and coupled with chlorpyrifos-BSA. Flow cytometry was then used to perform an indirect competitive immunoassay to detect chlorpyrifos residue. CBA is based on the use of three-dimensional magnetic microspheres and allophycocyanin-conjugated goat-anti-mouse secondary antibody (APC-IgG-SecAb), effectively avoiding interfering from the matrix. The results showed that this assay requires only 15 s for a single run. Furthermore, CBA was shown to have a detection limit of 1.09 ng mL−1and an IC50 of 15.95 ng mL−1. This detection limit value is approximately three times lower than that of ELISA, and the working range for chlorpyrifos detection covers three orders of magnitude. This study indicates that CBA method is a promising detection method with widespread utility for food safety and environmental monitoring.

中文翻译：

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使用磁性微球检测毒死蜱的灵敏细胞计数微球阵列

摘要 毒死蜱是一种广泛用于农业的有机磷农药，已知会造成环境污染，并对人类健康产生负面影响。在这项研究中，我们报道了一种检测毒死蜱的细胞计数微珠阵列 (CBA) 方法。制备磁性微球（Fe3O4@SiO2COOH）并与毒死蜱-BSA偶联。然后使用流式细胞术进行间接竞争性免疫测定以检测毒死蜱残留。CBA基于使用三维磁性微球和别藻蓝蛋白偶联的山羊抗小鼠二抗（APC-IgG-SecAb），有效避免来自基质的干扰。结果表明，该检测单次运行仅需 15 秒。此外，CBA 的检测限为 1.09 ng mL-1，IC50 为 15.95 ng mL-1。该检测限比ELISA低约三倍，毒死蜱检测的工作范围涵盖三个数量级。本研究表明，CBA 方法是一种很有前途的检测方法，在食品安全和环境监测方面具有广泛的用途。