当前位置：
X-MOL 学术
›
Chem. Phys. Lipids
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Apolipophorin III interaction with phosphatidylglycerol and lipopolysaccharide: A potential mechanism for antimicrobial activity.
Chemistry and Physics of Lipids ( IF 3.4 ) Pub Date : 2020-03-21 , DOI: 10.1016/j.chemphyslip.2020.104909 Eugenia Maravilla 1 , Duc P Le 1 , Jesse J Tran 1 , Michael H Chiu 2 , Elmar J Prenner 2 , Paul M M Weers 1
Chemistry and Physics of Lipids ( IF 3.4 ) Pub Date : 2020-03-21 , DOI: 10.1016/j.chemphyslip.2020.104909 Eugenia Maravilla 1 , Duc P Le 1 , Jesse J Tran 1 , Michael H Chiu 2 , Elmar J Prenner 2 , Paul M M Weers 1
Affiliation
|
Apolipophorin III (apoLp-III) is a model insect apolipoprotein to study structure-function relationships of exchangeable apolipoproteins. The protein associates with lipoproteins to aid in the transport of neutral lipids, and also interacts with the bacterial membrane. To better understand a potential role as an antimicrobial protein, the binding interaction of apoLp-III from Locust migratoria and Galleria mellonella with phosphatidylglycerol and lipopolysaccharides was analyzed. ApoLp-III from either species induced a robust release of calcein from phosphatidylglycerol vesicles, but was ineffective for phosphatidylcholine vesicles with comparable side-chain architecture. Acetylation of L. migratoria apoLp-III lysine residues greatly reduced the calcein release from phosphatidylglycerol vesicles, indicating a critical role of lysine side-chains in phosphatidylglycerol vesicles interaction. Isothermal calorimetry provided Kd values of 0.26 μM (L. migratoria) and 0.50 μM (G. mellonella) for binding to dimyristoylphosphatidylglycerol vesicles, which is an order of magnitude stronger compared to zwitterionic vesicles. A strong preference of apoLp-III for dimyristoylphosphatidylglycerol vesicles was also observed with differential scanning calorimetry with a concentration dependent shift in the lipid phase transition temperature. Native PAGE analysis showed that LPS binding was significantly weaker for L. migratoria apoLp-III compared to G. mellonella apoLp-III. This difference was confirmed by fluorescence titration analysis of L. migratoria apoLp-III, which also indicated that acetylation of the apolipoprotein did not affect LPS binding. Taken together, the results indicate that apoLp-III phosphatidylglycerol interaction may follow a detergent model with an important electrostatic binding component. Since lipopolysaccharide binding was not affected by neutralization of apoLp-III lysine-side chains, the binding interaction may be distinctly different from that of phosphatidylglycerol.
中文翻译：
载脂蛋白III与磷脂酰甘油和脂多糖的相互作用：抗菌活性的潜在机制。
载脂蛋白III（apoLp-III）是一种模型昆虫载脂蛋白，用于研究可交换载脂蛋白的结构-功能关系。该蛋白质与脂蛋白结合以帮助中性脂质的运输，并且还与细菌膜相互作用。为了更好地理解作为抗菌蛋白的潜在作用，分析了来自蝗虫和蜜蜂圆盘菌的apoLp-III与磷脂酰甘油和脂多糖的结合相互作用。两种物种的ApoLp-III均可从磷脂酰甘油囊泡中强烈释放钙黄绿素，但对于具有类似侧链结构的磷脂酰胆碱囊泡则无效。偏头痛乳酸杆菌apoLp-III赖氨酸残基的乙酰化大大降低了磷脂酰甘油囊泡中钙黄绿素的释放，表明赖氨酸侧链在磷脂酰甘油囊泡相互作用中的关键作用。等温量热法提供的Kd值分别为0.26μM（L. migratoria）和0.50μM（G. mellonella），以结合二肉豆蔻酰基磷脂酰甘油囊泡，这比两性离子囊泡要强一个数量级。用差示扫描量热法还观察到apoLp-III对二肉豆蔻酰基磷脂酰甘油囊泡的强烈偏爱，其中脂质相转变温度具有浓度依赖性的变化。天然PAGE分析表明，与黑麦草apoLp-III相比，L. migratoria apoLp-III的LPS结合明显弱。这种差异通过偏头痛乳杆菌apoLp-III的荧光滴定分析得到了证实，该分析还表明载脂蛋白的乙酰化不会影响LPS的结合。两者合计，结果表明apoLp-III磷脂酰甘油相互作用可能遵循具有重要静电结合成分的去污剂模型。由于脂多糖结合不受apoLp-III赖氨酸侧链的中和影响，因此结合相互作用可能与磷脂酰甘油明显不同。
更新日期：2020-03-22
中文翻译：
载脂蛋白III与磷脂酰甘油和脂多糖的相互作用：抗菌活性的潜在机制。
载脂蛋白III（apoLp-III）是一种模型昆虫载脂蛋白，用于研究可交换载脂蛋白的结构-功能关系。该蛋白质与脂蛋白结合以帮助中性脂质的运输，并且还与细菌膜相互作用。为了更好地理解作为抗菌蛋白的潜在作用，分析了来自蝗虫和蜜蜂圆盘菌的apoLp-III与磷脂酰甘油和脂多糖的结合相互作用。两种物种的ApoLp-III均可从磷脂酰甘油囊泡中强烈释放钙黄绿素，但对于具有类似侧链结构的磷脂酰胆碱囊泡则无效。偏头痛乳酸杆菌apoLp-III赖氨酸残基的乙酰化大大降低了磷脂酰甘油囊泡中钙黄绿素的释放，表明赖氨酸侧链在磷脂酰甘油囊泡相互作用中的关键作用。等温量热法提供的Kd值分别为0.26μM（L. migratoria）和0.50μM（G. mellonella），以结合二肉豆蔻酰基磷脂酰甘油囊泡，这比两性离子囊泡要强一个数量级。用差示扫描量热法还观察到apoLp-III对二肉豆蔻酰基磷脂酰甘油囊泡的强烈偏爱，其中脂质相转变温度具有浓度依赖性的变化。天然PAGE分析表明，与黑麦草apoLp-III相比，L. migratoria apoLp-III的LPS结合明显弱。这种差异通过偏头痛乳杆菌apoLp-III的荧光滴定分析得到了证实，该分析还表明载脂蛋白的乙酰化不会影响LPS的结合。两者合计，结果表明apoLp-III磷脂酰甘油相互作用可能遵循具有重要静电结合成分的去污剂模型。由于脂多糖结合不受apoLp-III赖氨酸侧链的中和影响，因此结合相互作用可能与磷脂酰甘油明显不同。
京公网安备 11010802027423号