Abstract In a previous study, the frequency of three different trypsin genotypes of Penaeus vannamei in aquaculture was described. Genotype CBA was the most abundant, followed by genotype CB, but genotype CA decreased its frequency and was not found in shrimps higher than 5 g of body weight. The conclusion was that shrimp with genotype CA inexplicably die under culture conditions. The question was if the process of shrimp aquaculture exerts excess pressure on the CA organisms, will they die? or will they die no matter where they grow?. To answer this question, the trypsin genotype frequencies in 60 breeder shrimp of a natural population were analyzed to predict the trypsin genotype frequency in the next generation. Then, a total of 1000 P. vannamei shrimp within the F1 generation of the same natural population weighing 2, 3, 5, 7 and 15 g were collected for 5 months, and the trypsin genotype frequencies were recorded. The results indicate that the F1 is out of Hardy–Weinberg equilibrium, and the absence of genotype CA in the natural population is similar to that in aquaculture. In conclusion, the process of aquaculture does not exert pressure on the CA genotype; such mortality is associated with another phenomenon not yet understood and deserving of further examination. At the moment, we know that the mortality occurs when shrimp inherit a trypsin codominant allele monomorphic called A, and the absence of allele B, and thus, we named the phenomenon “NON-B disruption”.

中文翻译：

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由于缺乏胰蛋白酶等位基因，在南美白对虾中观察到的新破坏

摘要 在先前的研究中，描述了南美白对虾三种不同胰蛋白酶基因型在水产养殖中的频率。基因型CBA最丰富，其次是基因型CB，但基因型CA降低其频率，在体重超过5g的虾中未发现。结论是基因型CA的虾在养殖条件下莫名其妙地死亡。问题是如果对虾养殖过程对CA生物施加过大的压力，它们会死亡吗？或者无论它们在哪里生长都会死亡？为了回答这个问题，我们分析了自然种群的 60 只种虾的胰蛋白酶基因型频率，以预测下一代的胰蛋白酶基因型频率。然后，在同一自然种群的 F1 代内总共 1000 只南美白对虾，体重分别为 2、3、5、5个月采集7g和15g，记录胰蛋白酶基因型频率。结果表明，F1 脱离了Hardy-Weinberg 平衡，自然种群中基因型CA 的缺失与水产养殖中相似。综上所述，水产养殖过程对CA基因型没有施加压力；这种死亡率与另一种尚未被了解并值得进一步研究的现象有关。目前，我们知道当虾继承了称为 A 的胰蛋白酶共显性等位基因单态性和等位基因 B 的缺失时会发生死亡，因此我们将这种现象命名为“NON-B 中断”。自然种群中CA基因型的缺失与水产养殖相似。综上所述，水产养殖过程对CA基因型没有施加压力；这种死亡率与另一种尚未被了解并值得进一步研究的现象有关。目前，我们知道当虾继承了称为 A 的胰蛋白酶共显性等位基因单态性和等位基因 B 的缺失时会发生死亡，因此我们将这种现象命名为“NON-B 中断”。自然种群中CA基因型的缺失与水产养殖相似。综上所述，水产养殖过程对CA基因型没有施加压力；这种死亡率与另一种尚未被了解并值得进一步研究的现象有关。目前，我们知道当虾继承了称为 A 的胰蛋白酶共显性等位基因单态性和等位基因 B 的缺失时会发生死亡，因此我们将这种现象命名为“NON-B 中断”。