Candida rugosa lipase (CRL) was treated with surfactants and immobilized onto the novel formulated magnetic graphene anchored silica nanocomposite (Fe₃O₄/SiO₂/Gr NC). For this purpose, the surface of lipase was initially coated with Triton-X 100 and cetyltrimethylammonium bromide surfactants, to stabilize enzyme in its open form and was then adsorbed onto aminated Fe₃O₄/SiO₂/Gr NC. Glutaraldehyde (GA) was then utilized to cross-link the adsorbed lipase onto the NC. The fabricated NC and conjugated lipase was characterized by various techniques such as FT-IR, XRD, TGA, SEM, TEM, CLSM, CD and Fluorescence spectroscopy. The magnetic character of the as-synthesized NC was verified by AGM investigation. CD and fluorescence spectroscopic analysis demonstrated slight structural rearrangements in lipase upon conjugation. The surfactant stabilized immobilized lipase demonstrated significantly enhanced thermostability, tolerance to various metal ions and inhibitors. The immobilization yield obtained owing to lipase interfacial activation by Triton X 100 and CTAB was remarkably enhanced by 6-folds and 3-folds, respectively which were remarkably higher in comparison to free immobilized lipase. The fabricated nanobiocatalysts were employed to synthesise green apple flavour ester, ethyl valerate via esterification reaction. Triton X 100 stabilized immobilized lipase was a better performer in yielding green apple flavour ester, demonstrating about 90% ester yield as compared to 78% yield obtained by CTAB stabilized immobilized lipase preparation. The obtained outcomes suggested that enzyme structure was stabilized by the GA treatment if executed in the absence or in the presence of detergent, and that, in the company of detergent, a conformation of the lipase with the exposed active center to the medium provided an aggrandized catalytic performance.

用表面活性剂处理皱念珠菌脂肪酶（CRL），并将其固定在新型配制的磁性石墨烯锚定的二氧化硅纳米复合材料（Fe ₃ O ₄ / SiO ₂ / Gr NC）上。为此目的，首先用Triton-X 100和十六烷基三甲基溴化铵表面活性剂涂覆脂肪酶表面，以使酶稳定成开放形式，然后吸附到胺化的Fe ₃ O ₄ / SiO _2上。/ Gr NC。然后利用戊二醛（GA）将吸附的脂肪酶交联到NC上。通过多种技术，如FT-IR，XRD，TGA，SEM，TEM，CLSM，CD和荧光光谱对制造的NC和共轭脂肪酶进行了表征。通过AGM研究验证了合成后NC的磁性。CD和荧光光谱分析显示，缀合后脂肪酶中的轻微结构重排。表面活性剂稳定的固定化脂肪酶表现出显着增强的热稳定性，对各种金属离子和抑制剂的耐受性。由于Triton X 100和CTAB对脂肪酶的界面活化作用而获得的固定化产率分别显着提高了6倍和3倍，与游离的固定化脂肪酶相比明显更高。利用所制备的纳米生物催化剂通过酯化反应合成了苹果青味酯，戊酸乙酯。Triton X 100稳定化的固定化脂肪酶在生产青苹果风味酯方面表现更好，与CTAB稳定化的固定化脂肪酶制备所获得的78％的收率相比，其酯收率约为90％。获得的结果表明，如果在不存在洗涤剂的情况下或在存在洗涤剂的情况下进行酶处理，则通过GA处理可稳定酶的结构，并且在洗涤剂公司的指导下，脂酶与活性中心暴露于介质中的构象提供了强化作用催化性能。Triton X 100稳定化的固定化脂肪酶在生产青苹果风味酯方面表现更好，与CTAB稳定化的固定化脂肪酶制备所获得的78％的收率相比，其酯收率约为90％。获得的结果表明，如果在不存在洗涤剂的情况下或在存在洗涤剂的情况下进行酶处理，则通过GA处理可稳定酶的结构，并且在洗涤剂公司的指导下，脂酶与活性中心暴露于介质中的构象提供了强化作用催化性能。Triton X 100稳定化的固定化脂肪酶在生产青苹果风味酯方面表现更好，与CTAB稳定化的固定化脂肪酶制备所获得的78％的收率相比，其酯收率约为90％。获得的结果表明，如果在不存在洗涤剂的情况下或在存在洗涤剂的情况下进行酶处理，则通过GA处理可稳定酶的结构，并且在洗涤剂公司的指导下，脂酶与活性中心暴露于介质中的构象提供了强化作用催化性能。