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Interleukin-18 promoter -137 G/C polymorphism (rs187238) is associated with biochemical markers of renal function and cardiovascular disease in type 2 diabetes patients.
Clinical Biochemistry ( IF 2.5 ) Pub Date : 2020-03-21 , DOI: 10.1016/j.clinbiochem.2020.03.011 Jânio Emanuel Andrade Cavalcante 1 , Ederson Laurindo Holanda de Sousa 1 , Raphael de Oliveira Rodrigues 1 , Glautemberg de Almeida Viana 1 , Daniel Duarte Gadelha 2 , Manoela Montenegro Dias de Carvalho 2 , Duaran Lopes Sousa 1 , Allysson Jordan Xavier Silva 1 , Raimundo Rigoberto Barbosa Xavier Filho 1 , Virgínia Oliveira Fernandes 2 , Renan Magalhães Montenegro Júnior 2 , Renata de Sousa Alves 1 , Gdayllon Cavalcante Meneses 3 , Tiago Lima Sampaio 1 , Maria Goretti Rodrigues Queiroz 1
Clinical Biochemistry ( IF 2.5 ) Pub Date : 2020-03-21 , DOI: 10.1016/j.clinbiochem.2020.03.011 Jânio Emanuel Andrade Cavalcante 1 , Ederson Laurindo Holanda de Sousa 1 , Raphael de Oliveira Rodrigues 1 , Glautemberg de Almeida Viana 1 , Daniel Duarte Gadelha 2 , Manoela Montenegro Dias de Carvalho 2 , Duaran Lopes Sousa 1 , Allysson Jordan Xavier Silva 1 , Raimundo Rigoberto Barbosa Xavier Filho 1 , Virgínia Oliveira Fernandes 2 , Renan Magalhães Montenegro Júnior 2 , Renata de Sousa Alves 1 , Gdayllon Cavalcante Meneses 3 , Tiago Lima Sampaio 1 , Maria Goretti Rodrigues Queiroz 1
Affiliation
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BACKGROUND
Interleukin-18 (IL-18), a proinflammatory and proatherogenic cytokine, has been associated with type 2 diabetes, metabolic syndrome, stroke and coronary artery disease. Some studies have indicated that the IL-18 promoter -137 G/C polymorphism seems to be associated with changes in the IL-18 expression and may contribute to the development of cardiovascular disease (CVD). The aim of this study was to evaluate the association between -137 G/C polymorphism and the levels of IL-18, biochemical markers for cardiovascular disorders, anthropometric profile and cardiovascular disease in Brazilian patients with type 2 diabetes (T2DM).
DESIGN & METHODS
Study subjects comprised 125 T2DM patients undergoing follow-up at a reference endocrinology service in northeastern Brazil. The -137G/C polymorphism in the IL-18 gene and serum IL-18 levels were determined by using allele-specific polymerase chain reaction (PCR) and enzyme-linked immune assay (ELISA), respectively. The anthropometric parameters were assessed using a Body Composition Monitor with Scale, and the laboratory data were measured using an automatic analyzer as well as spectrophotometric analysis.
RESULTS
The genotype distribution of IL-18 -137 G/C genetic polymorphism was significantly different among T2DM patients with and without CVD. The results show an association between the CC genotype of -137G/C polymorphism and CVD in T2DM patients (p < 0.001). Serum levels of IL-18 were significantly higher in CC carriers (843.1 pg/mL) compared with GG or GC carriers (303.6 pg/mL and 292.0 pg/mL, respectively). In addition, the present study showed that carriers of the CC genotype also had significantly higher concentrations of creatinine and albuminuria than carriers of the GG or GC genotypes (p < 0.05 in both).
CONCLUSION
These results suggest that Brazilian T2DM patients with the CC genotype seem to show a predisposition to CVD, as well as an elevation in markers of renal function.
中文翻译：
白介素-18启动子-137 G / C多态性（rs187238）与2型糖尿病患者肾功能和心血管疾病的生化标志物相关。
背景技术白细胞介素18（IL-18）是促炎和促动脉粥样硬化的细胞因子，已经与2型糖尿病，代谢综合征，中风和冠状动脉疾病相关。一些研究表明，IL-18启动子-137 G / C多态性似乎与IL-18表达的变化有关，并且可能有助于心血管疾病（CVD）的发展。这项研究的目的是评估巴西2型糖尿病（T2DM）患者的-137 G / C多态性与IL-18水平，心血管疾病的生化标志物，人体测量学特征和心血管疾病之间的相关性。设计与方法研究对象包括125名T2DM患者，他们在巴西东北部的参考内分泌服务处接受随访。IL-18基因和血清IL-18水平的-137G / C多态性分别通过等位基因特异性聚合酶链反应（PCR）和酶联免疫测定（ELISA）确定。使用带有刻度的人体成分监测仪评估人体测量学参数，并使用自动分析仪以及分光光度分析法测量实验室数据。结果在有和没有CVD的T2DM患者中，IL-18 -137 G / C基因多态性的基因型分布存在显着差异。结果显示，T2DM患者的-137G / C多态性CC基因型与CVD之间存在相关性（p <0.001）。与GG或GC载体（分别为303.6 pg / mL和292.0 pg / mL）相比，CC载体的血清IL-18水平显着更高（分别为843.1 pg / mL）。此外，本研究表明，CC基因型携带者的肌酐和蛋白尿浓度也明显高于GG或GC基因携带者（两者均p <0.05）。结论这些结果表明，巴西具有CC基因型的T2DM患者似乎显示出CVD的易感性，以及肾功能标志物的升高。
更新日期：2020-03-21
中文翻译：
白介素-18启动子-137 G / C多态性（rs187238）与2型糖尿病患者肾功能和心血管疾病的生化标志物相关。
背景技术白细胞介素18（IL-18）是促炎和促动脉粥样硬化的细胞因子，已经与2型糖尿病，代谢综合征，中风和冠状动脉疾病相关。一些研究表明，IL-18启动子-137 G / C多态性似乎与IL-18表达的变化有关，并且可能有助于心血管疾病（CVD）的发展。这项研究的目的是评估巴西2型糖尿病（T2DM）患者的-137 G / C多态性与IL-18水平，心血管疾病的生化标志物，人体测量学特征和心血管疾病之间的相关性。设计与方法研究对象包括125名T2DM患者，他们在巴西东北部的参考内分泌服务处接受随访。IL-18基因和血清IL-18水平的-137G / C多态性分别通过等位基因特异性聚合酶链反应（PCR）和酶联免疫测定（ELISA）确定。使用带有刻度的人体成分监测仪评估人体测量学参数，并使用自动分析仪以及分光光度分析法测量实验室数据。结果在有和没有CVD的T2DM患者中，IL-18 -137 G / C基因多态性的基因型分布存在显着差异。结果显示，T2DM患者的-137G / C多态性CC基因型与CVD之间存在相关性（p <0.001）。与GG或GC载体（分别为303.6 pg / mL和292.0 pg / mL）相比，CC载体的血清IL-18水平显着更高（分别为843.1 pg / mL）。此外，本研究表明，CC基因型携带者的肌酐和蛋白尿浓度也明显高于GG或GC基因携带者（两者均p <0.05）。结论这些结果表明，巴西具有CC基因型的T2DM患者似乎显示出CVD的易感性，以及肾功能标志物的升高。
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