Graphene-based transistors are promising devices in the evaluation of carrier density in biological analytes. We report on the design and fabrication of a graphene-based field-effect transistor for monitoring and assessing the interaction between the coagulation factors based on the charge carrier density in a blood sample. When biochemical reactions occurred during the coagulation cascade process, a dopant effect was noticed on the graphene surface by the change in Dirac point voltage values. Additional experiments were performed using blood samples treated with activators (vitamin K, calcium chloride, and thromboplastin reagent) and inhibitors (heparin drugs) to evaluate the selectivity of the graphene field-effect transistor devices. Since the transfer characteristic curves presented divergent behaviours for different levels of procoagulants and anticoagulants, the measurements showed that the devices can assess changes in the concentrations of factors that inhibit or accelerate the cascade process when using untreated and treated samples. Reproducibility was verified by testing samples from different sources. To the best of our knowledge, this study is the first to demonstrate the potential of graphene in monitoring the hemostasis process through the analysis of the electrical properties of human whole blood.

基于石墨烯的晶体管是评估生物分析物中载流子密度的有前途的设备。我们报告了基于石墨烯的场效应晶体管的设计和制造，该晶体管用于监测和评估基于血液样本中载流子密度的凝血因子之间的相互作用。当在凝血级联过程中发生生化反应时，通过狄拉克点电压值的变化在石墨烯表面上发现了掺杂效应。使用活化剂（维生素K，氯化钙和促凝血酶原酶试剂）和抑制剂（肝素药物）处理过的血液样本进行了其他实验，以评估石墨烯场效应晶体管器件的选择性。由于转移特性曲线在不同水平的促凝剂和抗凝剂上表现出不同的行为，因此测量结果表明，该设备可以评估使用或未处理样品时抑制或加速级联过程的因子浓度变化。通过测试不同来源的样品来验证可重复性。据我们所知，这项研究是第一个通过分析人类全血的电特性来证明石墨烯在监测止血过程中的潜力。