This study aimed to investigate the effects of laminin (LN) located in the basal lamina, which are important components of the peripheral nervous system‐extracellular matrix, on axon regeneration and remyelination. Nerve acellular scaffolds (NASs) (S‐untreated) were prepared using the acellular technique. The active component LN in the NASs was blocked (S‐LN⁻) or upregulated (S‐LN⁺); S‐LN+ contained seven times more LN than did the S‐untreated group. The adhesion capacity of Schwann cells (SCs) to the three types of NAS (S‐untreated, S‐LN⁻, and S‐LN⁺) was assessed in vitro. Our results showed that the adhesion of SCs to the NASs was significantly reduced in the S‐LN⁻ group, whereas no difference was observed between the S‐LN⁺ and S‐untreated groups. The pretreated NASs were used to repair nerves in a nerve injury mouse model with the animals divided into four groups (S‐LN⁻ group, S‐untreated group, S‐LN⁺ group, and autograft group). Two weeks after surgery, although there was no difference in the S‐LN⁻ group, S‐untreated group and S‐LN⁺ group, the newly formed basal lamina in the S‐LN⁻ group were significantly lower than those in the other two groups. Four weeks after surgery, the S‐LN⁺ group had higher numbers of newly generated axons and their calibers, more myelinated fibers, thicker myelin sheaths, increased myelin basic protein expression, and improved recovery of neural function compared to those of the S‐LN⁻ and S‐untreated groups, but all of these parameters were significantly worse than those of the autograft group. Downregulation of the LN level in the NAS leads to a reduction in all of the above parameters.

中文翻译：

---

使用神经脱细胞支架研究存在于周围神经系统基底层中的层粘连蛋白对轴突再生和髓鞘再生的影响。

本研究旨在研究位于基底层的层粘连蛋白（LN）对轴突再生和髓鞘再生的影响，基底层是周围神经系统 - 细胞外基质的重要组成部分。使用脱细胞技术制备神经脱细胞支架（NASs）（未经 S 处理）。在NASS的活性组分LN被阻断（S-LN ^- ）或上调（S-LN ⁺）; S-LN+ 所含的 LN 是 S-未治疗组的 7 倍。体外评估雪旺氏细胞（SCs）对三种类型的 NAS（S-未处理、S-LN ^-和 S-LN ⁺）的粘附能力。我们的结果表明，SCs 与 NASs 的粘附在 S-LN 中显着降低^-组，而在 S-LN ⁺和 S-未治疗组之间没有观察到差异。经预处理的NAS的被用来修复神经与分成四组（S-LN动物神经损伤小鼠模型^-基团，S-未处理组，S-LN ⁺基团，和自体移植物组）。手术后两周，虽然没有在S-LN没有区别^-组，S-模型组和S-LN ⁺组，在S-LN新成立的基底层^-组比其他两个分别显著降低组。手术后 4 周，S-LN ⁺与 S-LN ^-和 S-未处理组相比，组具有更多数量的新生成轴突及其口径、更多有髓纤维、更厚的髓鞘、增加的髓鞘碱性蛋白表达和改善的神经功能恢复，但所有这些参数明显低于自体移植组。NAS 中 LN 水平的下调导致上述所有参数的减少。