The extracellular matrix provides cells with a support structure and an attachment site in actual substrate. Its biochemical and surface properties play an important role in and have significant impact on cell attachment, proliferation, migration, differentiation, and gene expression. Leveraging the hydrophilicity and neuroprotective of gastrodin, a gastrodin/polyurethane (PU) elastomer was developed utilizing in situ polymerization and salt‐leaching methods. The results showed that gastrodin/PU film had a good flexibility and supporting strength, as well as hydrophilicity. Thus film possessed highly surface area, interconnected porous structure with a pore size (10~60 μm) for cell attachment, and could provide surface cues to augment neurite extension. For PC12 cells cultured within the films, especially the 5gastrodin/PU group, presented a progressive increase with time, coupled with the upregulation of brain‐derived neurotrophic factor and glial cell derived neurotrophic factor expression. This is the first report on the construction of a gastrodin/PU porous film, and the results reveal its promise as a scaffold material for neural tissue engineering.

中文翻译：

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用天麻素功能化的弹性聚氨酯多孔膜用于外周神经再生。

细胞外基质为细胞提供了支撑结构和实际基质中的附着位点。其生化和表面特性在细胞附着、增殖、迁移、分化和基因表达中起着重要作用并具有显着影响。利用天麻素的亲水性和神经保护作用，利用原位聚合和盐浸方法开发了天麻素/聚氨酯（PU）弹性体。结果表明，天麻素/PU薄膜具有良好的柔韧性和支撑强度，以及亲水性。因此，薄膜具有高表面积、具有孔径（10~60 μm）的互连多孔结构用于细胞附着，并且可以提供表面线索以增加神经突延伸。对于在膜内培养的 PC12 细胞，尤其是 5gastrodin/PU 组，随着时间的推移呈现逐渐增加，加上脑源性神经营养因子和神经胶质细胞源性神经营养因子表达的上调。这是关于构建天麻素/PU 多孔膜的第一份报告，结果显示其作为神经组织工程支架材料的前景。