Adjustment of Cell Adhesion on Polyurethane Structures via Control of the Hard/Soft Segment Ratio,Macromolecular Materials and Engineering

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Adjustment of Cell Adhesion on Polyurethane Structures via Control of the Hard/Soft Segment Ratio
Macromolecular Materials and Engineering ( IF 4.2 ) Pub Date : 2020-03-20 , DOI: 10.1002/mame.202000093
Gaëtan Lutzweiler ₁ , Julien Barthes ₁ , Nihal Engin Vrana ₂ , Michel Rawiso ₃ , Benoît Louis ₄ , Josselin Mayingi ₅ , Albane Carre ₁ , Wiebke Drenckhan ₃ , Pierre Schaaf ₁

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Creating substrates with a similar composition that can either prevent or promote cell adhesion is still a challenging feat. Here, it is shown that a strikingly simple method of tuning the amount of hard segments or isocyanate index (NCO_ind) of a polyurethane (PU) film allows to modulate cell adhesiveness. PU films are synthesized with NCO_ind of 75, 100, 200, 300 and 400 corresponding to ratios of isocyanate to hydroxyl functions of 0.75, 1, 2, 3, 4, respectively. The adhesive capacity of NIH 3T3 fibroblasts (3T3) and Wharton's jelly mesenchymal stem cells (WJMSCs) are dependent on the NCO_ind. For NCO_ind below 300, no cell adhesion can be observed regardless of the cell type, whereas for NCO_ind of 300 and 400 cells adhere to the PU surface. WAXS and small angle X‐ray scattering (SAXS) studies reveal that variations of NCO_ind allows to modulate the phase separation in PU films. Porod's law shows that for NCO_ind of 300 and 400, the hard–soft segment interface is sharp. Conversely, samples with smaller NCO_ind present diffuse interfaces. Hence, the morphology of the interface between hard and soft domains appears to be a critical feature that correlates with the adhesion capacity of cells.

中文翻译：

通过控制硬/软链段比来调节聚氨酯结构上的细胞粘附

创建具有相似成分的底物可以预防或促进细胞粘附仍然是一项艰巨的任务。在此表明，调整聚氨酯（PU）膜的硬链段或异氰酸酯指数（NCO _ind）的数量非常简单的方法可以调节细胞粘附性。合成具有_{75、100、200、300}和400的NCO _ind的PU膜，分别对应于异氰酸酯与羟基官能团的比分别为0.75、1、2、3、4。NIH 3T3成纤维细胞（3T3）和沃顿氏果冻间充质干细胞（WJMSCs）的粘附能力取决于NCO _ind。对于低于300的NCO _ind，无论细胞类型如何，都无法观察到细胞粘附，而对于NCO _ind300和400个单元中的100个粘附在PU表面上。WAXS和小角度X射线散射（SAXS）研究表明，NCO _ind的变化可以调节PU膜中的相分离。波罗德定律表明，对于300和400的NCO _ind，硬-软段界面很清晰。相反，具有较小NCO _ind的样品存在扩散界面。因此，硬结构域和软结构域之间的界面形态似乎是与细胞粘附能力相关的关键特征。

更新日期：2020-03-20

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