Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44.,Journal of Materials Chemistry B

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Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-05-06 , DOI: 10.1039/d0tb00407c
Sara Amorim ₁ , Iva Pashkuleva ₂ , Celso A Reis ₃ , Rui L Reis ₁ , Ricardo A Pires ₁

Affiliation

We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) - a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion.

中文翻译：

包含透明质酸及其与CD44相互作用的可调谐的逐层薄膜。

我们报道了层-层（LbL）结构的发展，该结构的粘弹性和生物活性可以通过使用不同大小和/或交联的聚阴离子进行精细调节。作为聚阴离子，我们使用了透明质酸（HA）-一种多信号生物分子，其生物活性取决于其分子量。我们调查了使用不同大小的HA构建的LbL系统的机械性能与特定的HA介导的生化相互作用之间的相互作用。我们通过耗散的石英晶体微天平（QCM-D），表面等离子体共振（SPR）和原子力显微镜（AFM）对组装的材料及其与主要HA受体CD44的相互作用进行了表征。我们观察到CD44的存在导致了非交联多层的破坏，

更新日期：2020-03-20

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