Several biosensors based on the immobilization of alkaline phosphatase (ALP) at different transducers have been reported in literature. In this work, a gold electrode covalently modified with a hydrogel (Au-HGL) was used as a platform for the immobilization of ALP enzyme to produce Au-HGL/ALP biosensor. The biosensor construction was evaluated by Raman spectroscopy, cyclic voltammetry (CV), and atomic force microscopy (AFM). The analytical performance of the biosensor was assessed by square wave voltammetry (SWV), electrochemical impedance spectroscopy (EIS), and amperometry. The calibration curve displayed a dynamic linear range of 0.5–30 μM with a detection limit (LOD) and quantification limit (LOQ) of 0.23 μM and 0.76 μM, respectively. Determination of vanadium in commercial Centrum® tablet using the proposed biosensor was satisfactory when compared to other analytical methods. The proposed Au-HGL/ALP biosensor exhibited high sensitivity to vanadium in an aqueous medium with a good reproducibility (n = 4) and a relative standard deviation (RSD) of 8.0%.

Graphical Abstract

在文献中已经报道了几种基于将碱性磷酸酶（ALP）固定在不同换能器上的生物传感器。在这项工作中，以水凝胶（Au-HGL）共价修饰的金电极用作固定ALP酶以生产Au-HGL / ALP生物传感器的平台。通过拉曼光谱，循环伏安法（CV）和原子力显微镜（AFM）评估生物传感器的结构。通过方波伏安法（SWV），电化学阻抗谱（EIS）和安培法评估生物传感器的分析性能。校准曲线显示的动态线性范围为0.5–30μM，检出限（LOD）和定量限（LOQ）分别为0.23μM和0.76μM。与其他分析方法相比，使用拟议的生物传感器测定商用Centrum®片剂中的钒是令人满意的。拟议的Au-HGL / ALP生物传感器在水性介质中对钒表现出高灵敏度，并具有良好的重现性（n  = 4），相对标准偏差（RSD）为8.0％。

图形概要