Parametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box–Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. The maximum lactic acid yield was obtained as 0.938 ± 0.003 g/g glucose with a productivity of 2.213 ± 0.008 g/(L × h) under the optimized conditions of 2.0 mm bead diameter, 5.60 pH, 115.3 g/L initial glucose concentration, and 398.2 mg biomass (CDW) in 100 mL hydrogel. The analysis of variance indicated that the quadratic model was significant and could be used to scale up the fermentation process.

中文翻译：

---

固定化乳酸杆菌发酵生产乳酸的优化。

参数优化是发酵过程中提高产品产量和生产率以节省时间，空间和财务资源的有效方法。在这项研究中，采用Box–Behnken设计来优化固定化戊糖乳杆菌ATCC 8041细胞发酵产生乳酸的条件。进行了两个二次模型和响应面分析，以说明固定化戊糖乳杆菌中各个参数及其相互作用对乳酸产量和葡萄糖消耗率的影响。ATCC 8041细胞发酵。在2.0 mm珠粒直径，5.60 pH，115.3 g / L初始葡萄糖浓度的优化条件下，最大乳酸产量为0.938±0.003 g / g葡萄糖，生产率为2.213±0.008 g /（L×h）。 100毫升水凝胶中的398.2毫克生物质（CDW）。方差分析表明，二次模型很重要，可用于扩大发酵过程。