Abstract

Cardiac arrhythmia is an irregular heart rhythm that can lead to serious heart conditions and various organ disorders, and may cause sudden cardiac death. Catalpol belongs to the iridoid glycoside family and is highly abundant in Rehmannia glutinosa Libosch. The study included five groups such as group I (normal control), group II (treatment control), group III (low-dose treatment), group IV (medium-dose treatment) and group V (high-dose treatment). We investigated the therapeutic effects of catalpol on cardiac arrhythmia in human-induced pluripotent stem cells (iPSCs). Cell viability, lactate dehydrogenase (LDH) levels, lipid peroxidation, antioxidant activity, and caspase-3 and caspase-9 activities and protein levels were measured in normal control, treatment control, and treated (1, 10, and 100 µM) iPSC groups. Compared with the treatment control group, catalpol supplementation (1, 10, and 100 µM) increased iPSC cell viability by 7.5, 27.3, and 65.8%, respectively; reduced the LDH levels by 10.4, 31.3, and 75.2%, respectively; and reduced the lipid peroxidation levels by 7.7, 33.0, and 62.6%, respectively. The antioxidant levels were significantly higher in the treatment control group than in the normal control group. Catalpol (100 µM) reduced the caspase-3 and caspase-9 activities by more than 30% and increased expression of the corresponding proteins by more than 50%. These findings suggest that the naturally occurring iridoid glycoside catalpol is effective against aconitine-induced cardiac arrhythmia in iPSCs.

中文翻译：

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梓醇对人诱导多能干细胞产生的心肌细胞凋亡的治疗作用。

摘要

心律失常是一种不规则的心律，可导致严重的心脏病和各种器官疾病，并可能导致心脏猝死。Catalpol属于鸢尾糖苷家族，在地黄中含量很高Libosch。该研究包括五个组，例如I组（正常对照组），II组（治疗对照组），III组（低剂量治疗），IV组（中剂量治疗）和V组（高剂量治疗）。我们调查了梓醇对人诱导的多能干细胞（iPSC）中的心律不齐的治疗作用。在正常对照组，治疗对照组和治疗组（1、10和100 µM）中，测量了细胞活力，乳酸脱氢酶（LDH）水平，脂质过氧化，抗氧化剂活性以及caspase-3和caspase-9活性以及蛋白质水平。 。与治疗对照组相比，添加梓醇（1、10和100 µM）分别使iPSC细胞活力提高了7.5、27.3和65.8％。将LDH水平分别降低10.4％，31.3％和75.2％；并将脂质过氧化水平降低了7.7、33.0和62。分别为6％。治疗对照组的抗氧化剂水平明显高于正常对照组。Catalpol（100 µM）使caspase-3和caspase-9活性降低了30％以上，相应蛋白质的表达提高了50％以上。这些发现表明，天然存在的环烯醚酮苷梓醇对iPSC中乌头碱引起的心律不齐有效。