Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM.

中文翻译：

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脂质组学和 RNA 测序揭示了细胞外基质生物材料中纳米囊泡的新亚群。


由细胞外基质 (ECM) 组成的生物材料提供机械支撑和促进功能性组织修复的建设性信号分子库。最近，基质结合纳米囊泡 (MBV) 已被报道为 ECM 生物支架的组成部分。尽管液相细胞外囊泡 (EV) 一直是深入研究的主题，但它们与 MBV 的相似之处仅限于大小和形状。基于液相色谱-质谱 (LC-MS) 的脂质组学和氧化还原脂质组学用于对液相 EV 和 MBV 磷脂进行详细比较。结合对囊泡内货物的全面 RNA 测序和生物信息学分析，我们表明 MBV 是 EV 的一个独特的亚群，也是基于 ECM 的生物材料的显着特征。结果开始确定由组织驻留细胞分泌并沉积在 ECM 内的 EV 介导的差异生物活性。