The possibility of using marine diatom-infecting viral promoters for the engineering of marine diatoms,Plant Science

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The possibility of using marine diatom-infecting viral promoters for the engineering of marine diatoms
Plant Science ( IF 4.2 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.plantsci.2020.110475
Takashi Kadono ₁ , Yuji Tomaru ₂ , Kengo Suzuki ₃ , Koji Yamada ₃ , Masao Adachi ₁

Affiliation

Marine diatoms constitute a major group of unicellular photosynthetic eukaryotes. Diatoms are widely applicable for both basic studies and applied studies. Molecular tools and techniques have been developed for diatom research. Among these tools, several endogenous gene promoters (e.g., the fucoxanthin chlorophyll a/c-binding protein gene promoter) have become available for expressing transgenes in diatoms. Gene promoters that drive transgene expression at a high level are very important for the metabolic engineering of diatoms. Various marine diatom-infecting viruses (DIVs), including both DNA viruses and RNA viruses, have recently been isolated, and their genome sequences have been characterized. Promoters from viruses that infect plants and mammals are widely used as constitutive promoters to achieve high expression of transgenes. Thus, we recently investigated the activity of promoters derived from marine DIVs in the marine diatom, Phaeodactylum tricornutum. We discuss novel viral promoters that will be useful for the future metabolic engineering of diatoms.

中文翻译：

使用感染海洋硅藻的病毒启动子进行海洋硅藻工程的可能性

海洋硅藻构成了单细胞光合真核生物的主要群体。硅藻广泛适用于基础研究和应用研究。已经开发出用于硅藻研究的分子工具和技术。在这些工具中，几种内源基因启动子（例如岩藻黄质叶绿素 a/c 结合蛋白基因启动子）已可用于在硅藻中表达转基因。高水平驱动转基因表达的基因启动子对于硅藻的代谢工程非常重要。最近分离了各种海洋硅藻感染病毒 (DIV)，包括 DNA 病毒和 RNA 病毒，并对其基因组序列进行了表征。来自感染植物和哺乳动物的病毒的启动子被广泛用作组成型启动子以实现转基因的高表达。因此，我们最近研究了源自海洋硅藻三角褐指藻中海洋 DIV 的启动子的活性。我们讨论了对未来硅藻代谢工程有用的新型病毒启动子。

更新日期：2020-07-01

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