Pyrolysis bio-oils are liquid products of lignocellulosic biomass pyrolysis. They have a highly promising potential to be widely used, after an appropriate upgrade, as advanced biofuels, or as a source of valuable oxygen-containing chemicals. The chemical composition of bio-oils is very complicated as they contain thousands of different, mostly oxygen-containing, compounds with a wide distribution of physical and chemical properties, and concentrations. Detailed knowledge of the bio-oil composition is crucial in order to optimize the pyrolysis processes and/or the subsequent bio-oil upgrading processes. The main challenge in bio-oil analytics is the identification and quantification of the individual compounds as well as the quantification of the total content of the compounds with the characteristic functional groups. In this review, we will discuss a state-of-the-art quantitative analysis of bio-oils and formulate strategies for obtaining in-depth information on the composition of the bio-oils and/or the products of their upgrading. Thermic, non-catalytic fast pyrolysis bio-oils and their hydrotreated analogues are of interest of this review. The emphasis will be placed on the quantification of the compounds with the key oxygen-containing functional groups present in the bio-oils including aldehydes, ketones, carboxylic acids, phenols, carbohydrates, etc. Also, methods for the quantification of the individual compounds will be presented. Hence, this overview and critical assessment of the quantitative methods can help the researchers to better understand the results obtained by these methods and formulate strategies and goals for further research. In addition, the knowledge presented in this review will serve as a reference to any scientist working with complex mixtures of oxygenates.

热解生物油是木质纤维素生物质热解的液体产物。在适当升级后，它们具有极高的潜力，可广泛用作先进的生物燃料或作为有价值的含氧化学品的来源。生物油的化学组成非常复杂，因为它们包含成千上万种具有广泛分布的物理和化学特性及浓度的化合物，大多数为含氧化合物。为了优化热解过程和/或随后的生物油提质过程，对生物油组成的详细了解至关重要。生物油分析中的主要挑战是单个化合物的鉴定和定量，以及具有特征官能团的化合物总含量的定量。在这篇评论中 我们将讨论生物油的最新定量分析，并制定策略以获取有关生物油和/或其提质产品的深入信息。热性，非催化性快速热解生物油及其加氢处理的类似物是该综述的重点。重点将放在具有生物油（包括醛，酮，羧酸，酚，碳水化合物等）中存在的关键含氧官能团的化合物的定量上。此外，单个化合物的定量方法也将被提出。因此，对定量方法的概述和批判性评估可以帮助研究人员更好地理解通过这些方法获得的结果，并制定进一步研究的策略和目标。此外，