Sepiapterin reductase deficiency (SR) is a rare inborn disorder of neurotransmitter metabolism. The early diagnosis of SR disease should be achieved through the determination of the sepiapterin level in body fluids of suspected patients. Here, we report the selection, identification, and characterization of DNA aptamers against sepiapterin. The aptamer selection was achieved via the systematic evolution of ligand by the exponential enrichment technique. After ten rounds of selection, high-affinity aptamers were identified. The binding affinities of the selected aptamers were evaluated using fluorescence binding assays showing dissociation constants ranging from 37.3 to 79.0 nM. The highest affinity aptamer was then integrated into a competitive electrochemical biosensor. The biosensor achieved outstanding sensitivity with a detection limit of 0.8 pg/ml which was much lower than the reported chromatographic method for sepiapterin quantification. The aptasensor has also shown a high degree of selectivity against the closely-related compound. The aptasensor was then challenged by detecting the sepiapterin in spiked serum samples where a good recovery percentage was achieved.

Sepaapterin还原酶缺乏症（SR）是一种罕见的先天性神经递质代谢疾病。SR病的早期诊断应通过确定可疑患者体液中的Sepaapterin水平来实现。在这里，我们报告针对sepiapterin的DNA适体的选择，鉴定和表征。通过指数富集技术通过配体的系统进化来实现适体选择。经过十轮选择，鉴定出高亲和力的适体。使用显示出解离常数为37.3至79.0 nM的解离常数的荧光结合测定法评估所选适体的结合亲和力。然后将最高亲和力的适体整合到竞争性电化学生物传感器中。该生物传感器具有出色的灵敏度，检测极限为0。8 pg / ml，远低于报道的Sepaapterin定量色谱方法。适体传感器还显示出对紧密相关化合物的高度选择性。然后通过检测加标血清样品中的Sepaapterin来挑战aptasensor，回收率很高。