A simple benzothizole-based derivative 2-(benzo[d]thiazol-2-yl)phenyl)picolinamide (BTPPA) with aggregation-induced emission (AIE) character has been developed. BTPPA is non-emissive in DMF, but displays strong fluorescence emission in DMF/H₂O (1:9, v/v, Tris-HCl 10 mM, pH = 7.1) solution due to its aggregation. The BTPPA aggregates exhibit highly selective recognition toward Cu²⁺ ions through formation of BTPPA-Cu²⁺ complex with a 1:1 binding stoichiometry, leading to disaggregation and complete fluorescence quenching. The in situ generated BTPPA-Cu²⁺ complex can further act as a chemosensing ensemble for highly selective recognition of pyrophosphate (PPi) via Cu²⁺ displacement approach, which results in re-aggregation of BTPPA and fluorescence recovery. Thus relay recognition of Cu²⁺ and PPi has been achieved with ‘on-off-on’ emission changes through aggregation-switching strategy. The limits of detection for Cu²⁺ and PPi were estimated to be 5.06 × 10⁻⁷ M and 4.73 × 10⁻⁶ M. In addition, detection of Cu²⁺ and PPi in real samples such as tea, fish, crab meat, toothpaste and detergent were also achieved with good recoveries and small relative standard deviations, demonstrating the potential practicability of BTPPA and BTPPA-Cu²⁺ for Cu²⁺ and PPi detection in real samples.

一个简单的基于苯并噻唑的衍生物2-（苯并[ d ]噻唑-2-基）苯基）吡啶啉酰胺（BTPPA）具有聚集诱导发射（AIE）特性。BTPPA在DMF中是非发射性的，但由于其聚集，在DMF / H ₂ O（1：9，v / v，Tris-HCl 10 mM，pH = 7.1）溶液中显示强荧光发射。所述BTPPA聚集体表现出对铜的高选择性识别²⁺离子通过形成BTPPA -Cu ²⁺复合物与1：1的结合化学计量，从而导致分解和完整的荧光淬灭。该原位生成的BTPPA -Cu ²⁺该复合物还可以充当化学传感集合体，以通过Cu ²⁺置换方法高度选择性地识别焦磷酸盐（PPi），从而导致BTPPA的重新聚集和荧光恢复。因此，通过聚集切换策略通过“开-关-开”发射变化实现了对Cu ²⁺和PPi的中继识别。Cu ²⁺和PPi的检出限估计为5.06×10 ^-7  M和4.73×10 ^-6M  。此外，Cu ^2+的检出茶叶，鱼，蟹肉，牙膏和清洁剂等真实样品中的PPi和PPi的回收率也很高，相对标准偏差也很小，证明了BTPPA和BTPPA- Cu ²⁺在实际检测Cu ²⁺和PPi方面的潜在实用性样品。