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Low cost Raman sample substrates from conductive silver paint smear for Raman spectroscopic screening of metabolic diseases in whole blood
Vibrational Spectroscopy ( IF 2.5 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.vibspec.2020.103063 Zephania Birech , Annah M. Ondieki , Robert I.I. Opati , Peter W. Mwangi
Vibrational Spectroscopy ( IF 2.5 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.vibspec.2020.103063 Zephania Birech , Annah M. Ondieki , Robert I.I. Opati , Peter W. Mwangi
Abstract This work reports on a low cost, simple to prepare and chemically stable Raman substrates based on conductive silver paint smear. The substrates were characterized Raman spectroscopically and were found to be chemically stable within the first seven days when kept at room temperature as the spectroscopic profiles were unchanged. The substrates also suppressed the background signals emanating from glass centered around 750 cm−1 and 1370 cm−1 seen with 785 nm excitation and had negligible influence on Raman spectral profiles of rat’s blood samples applied onto them. The Raman spectral profiles of blood samples applied onto the substrates were found to be enhanced by a factor of 1.7 compared to those of thick blood smears on a clean microscope glass slide. The increased local field between the gaps formed by adjacent micron-sized silver solids in the paint smear were attributed to the observed intense signals observed from the blood samples applied onto them. The substrates were tried on Raman spectroscopic differentiation between blood from obese and normal; diabetic and normal Sprague Dawley rats. The prominent bands associated with fructose (638 and 812 cm−1), glucose (1127 cm−1) and branched chain amino acids (1033, 1217 and 1318 cm−1) were observed to vary in terms of intensity between the un-healthy (obese and diabetic) and healthy (normal) rats. The results reported here on the use of the easy to prepare, low cost Raman substrates have the potential of making surface-enhanced Raman spectroscopy accessible to less resourced laboratories in developing countries. The substrates can be applied in rapid Raman spectroscopic screening of various metabolic diseases.
更新日期：2020-05-01
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