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Expansion of the Major Facilitator Superfamily (MFS) to include novel transporters as well as transmembrane-acting enzymes.
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2020-03-20 , DOI: 10.1016/j.bbamem.2020.183277
Steven C Wang 1 , Pauldeen Davejan 1 , Kevin J Hendargo 1 , Ida Javadi-Razaz 1 , Amy Chou 1 , Daniel C Yee 1 , Faezeh Ghazi 1 , Katie Jing Kay Lam 1 , Adam M Conn 1 , Assael Madrigal 1 , Arturo Medrano-Soto 1 , Milton H Saier 1
Affiliation  

The Major Facilitator Superfamily (MFS) is currently the largest characterized superfamily of transmembrane secondary transport proteins. Its diverse members are found in essentially all organisms in the biosphere and function by uniport, symport, and/or antiport mechanisms. In 1993 we first named and described the MFS which then consisted of 5 previously known families that had not been known to be related, and by 2012 we had identified a total of 74 families, classified phylogenetically within the MFS, all of which included only transport proteins. This superfamily has since expanded to 89 families, all included under TC# 2.A.1, and a few transporter families outside of TC# 2.A.1 were identified as members of the MFS. In this study, we assign nine previously unclassified protein families in the Transporter Classification Database (TCDB; http://www.tcdb.org) to the MFS based on multiple criteria and bioinformatic methodologies. In addition, we find integral membrane domains distantly related to partial or full-length MFS permeases in Lysyl tRNA Synthases (TC# 9.B.111), Lysylphosphatidyl Glycerol Synthases (TC# 4.H.1), and cytochrome b561 transmembrane electron carriers (TC# 5.B.2). Sequence alignments, overlap of hydropathy plots, compatibility of repeat units, similarity of complexity profiles of transmembrane segments, shared protein domains and 3D structural similarities between transport proteins were analyzed to assist in inferring homology. The MFS now includes 105 families.

中文翻译:

扩大主要促进剂超家族 (MFS) 以包括新型转运蛋白和跨膜作用酶。

主要促进剂超家族 (MFS) 是目前最大的表征跨膜二级转运蛋白超家族。其多样化的成员基本上存在于生物圈中的所有生物体中,并通过单向、同向和/或反向机制发挥作用。1993 年,我们首次命名和描述了 MFS,该 MFS 由 5 个以前已知的家族组成,但不知道是相关的,到 2012 年,我们总共确定了 74 个家族,在 MFS 内进行系统发育分类,所有这些家族仅包括运输蛋白质。此超家族已经扩展到 89 个家族,全部包含在 TC# 2.A.1 之下,并且一些 TC# 2.A.1 之外的转运蛋白家族被确定为 MFS 的成员。在这项研究中,我们在转运蛋白分类数据库 (TCDB; http: //www.tcdb.org) 到基于多个标准和生物信息学方法的 MFS。此外,我们发现与赖氨酰 tRNA 合酶 (TC# 9.B.111)、赖氨酰磷脂酰甘油合酶 (TC# 4.H.1) 和细胞色素 b561 跨膜电子中的部分或全长 MFS 通透酶远相关的完整膜结构域运营商(TC# 5.B.2)。分析了序列比对、亲水性图的重叠、重复单元的兼容性、跨膜片段复杂性特征的相似性、共享的蛋白质结构域和转运蛋白之间的 3D 结构相似性,以帮助推断同源性。MFS 现在包括 105 个家庭。我们发现与赖氨酰 tRNA 合酶 (TC# 9.B.111)、赖氨酰磷脂酰甘油合酶 (TC# 4.H.1) 和细胞色素 b561 跨膜电子载体 (TC) 中的部分或全长 MFS 通透酶远相关的完整膜结构域# 5.B.2)。分析了序列比对、亲水性图的重叠、重复单元的兼容性、跨膜片段复杂性特征的相似性、共享的蛋白质结构域和转运蛋白之间的 3D 结构相似性,以帮助推断同源性。MFS 现在包括 105 个家庭。我们发现与赖氨酰 tRNA 合酶 (TC# 9.B.111)、赖氨酰磷脂酰甘油合酶 (TC# 4.H.1) 和细胞色素 b561 跨膜电子载体 (TC) 中的部分或全长 MFS 通透酶远相关的完整膜结构域# 5.B.2)。分析了序列比对、亲水性图的重叠、重复单元的兼容性、跨膜片段复杂性特征的相似性、共享的蛋白质结构域和转运蛋白之间的 3D 结构相似性,以帮助推断同源性。MFS 现在包括 105 个家庭。分析了转运蛋白之间的共享蛋白结构域和 3D 结构相似性,以帮助推断同源性。MFS 现在包括 105 个家庭。分析了转运蛋白之间的共享蛋白结构域和 3D 结构相似性,以帮助推断同源性。MFS 现在包括 105 个家庭。
更新日期:2020-03-20
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