Bacillus amyloliquefaciens is a non-pathogenic and plant growth-promoting rhizobacterium that enhances plant resistance to drought and diseases. Arabidopsis thaliana is a multipurpose model plant for exploring microorganism-plant interactions and a crucial vegetal tool for molecular research. Non-coding RNAs are RNA molecules involved in the regulation of various biological functions and constitute a research hotspot in the field of plant biology. In this study, the effect of B. amyloliquefaciens treatment on the resistance of A. thaliana to high calcium stress was analyzed. The transcriptome sequencing of A. thaliana roots under four treatment conditions was performed to screen differentially expressed lncRNAs, mRNAs and miRNAs. Functional analysis was also performed to understand the potential mechanism by which B. amyloliquefaciens-regulated lncRNAs, miRNAs and mRNAs affect the resistance of A. thaliana to high calcium stress. The results indicated that B. amyloliquefaciens treatment increased the resistance of A. thaliana to high calcium stress. A set of differentially expressed lncRNAs, mRNAs and miRNAs were screened between the high calcium and control group on one hand, and high calcium and high calcium + B. amyloliquefaciens groups on the other hand. Functional analysis indicated that the differentially expressed mRNAs and miRNA were involved in various biological functions and that transcriptional dysregulation caused by high calcium stress involves metabolic processes rather than defense responses. Conclusively, B. amyloliquefaciens may improve the resistance of A. thaliana to high calcium stress via a lncRNA-miRNA-mRNA regulatory network. These findings will contribute to the development of agriculture in karst regions with high calcium content.

中文翻译：

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解淀粉芽孢杆菌LZ04提高拟南芥对高钙胁迫的抗性以及lncRNA-miRNA-mRNA调控网络在抗性中的潜在作用。

解淀粉芽孢杆菌是一种非病原性植物，可促进植物生长，可增强植物对干旱和疾病的抵抗力。拟南芥是一种用于探索微生物与植物相互作用的多用途模型植物，并且是进行分子研究的重要植物工具。非编码RNA是参与各种生物学功能调节的RNA分子，是植物生物学领域的研究热点。在这项研究中，分析了解淀粉芽孢杆菌对拟南芥对高钙胁迫抗性的影响。在四种处理条件下对拟南芥根进行转录组测序以筛选差异表达的lncRNA，mRNA和miRNA。还进行了功能分析以了解B的潜在机制。淀粉寡糖调节的lncRNA，miRNA和mRNA影响拟南芥对高钙胁迫的抗性。结果表明，解淀粉芽孢杆菌处理增加了拟南芥对高钙胁迫的抗性。一方面在高钙和对照组之间，另一方面在高钙和高钙+解淀粉芽孢杆菌组之间筛选了一组差异表达的lncRNA，mRNA和miRNA。功能分析表明差异表达的mRNA和miRNA参与了各种生物学功能，并且高钙胁迫引起的转录失调涉及代谢过程而不是防御反应。结论是，解淀粉芽孢杆菌可通过lncRNA-miRNA-mRNA调控网络提高拟南芥对高钙胁迫的抗性。