To prove that oxygen is irreversibly consumed in the blue bottle reaction, a manometer or a plastic bottle was used to show a permanent reduction in the gas pressure in a reaction vessel. This demonstration may be misleading because the change in gas pressure can be attributed to a number of other factors such as temperature change and involvement of other gases. More importantly, the use of a plastic bottle should be discontinued because of safety and environmental concerns. An inexpensive hand-held oxygen gas probe is employed instead to provide conclusive proof of irreversible oxygen consumption. The new procedure can also help reveal the role of the redox dye that was previously assumed to be a catalyst for all variations of the blue bottle reaction. Our improved procedure shows that, unlike sugar reducing (in classical version of the blue bottle reaction), benzoin and vitamin C (in rapid and green versions of the blue bottle reaction) undergo autoxidation at noticeable rates without the dye.

中文翻译：

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蓝瓶实验再探：多少氧气？

为了证明在蓝瓶反应中不可逆地消耗了氧气，使用了压力计或塑料瓶来显示反应容器中气压的永久降低。由于气体压力的变化可能归因于许多其他因素，例如温度变化和其他气体的参与，因此该演示可能会产生误导。更重要的是，出于安全和环境方面的考虑，应停止使用塑料瓶。取而代之的是，使用廉价的手持式氧气探针来提供不可逆的氧气消耗的确凿证据。新程序还可以帮助揭示氧化还原染料的作用，该氧化还原染料以前被认为是蓝瓶反应所有变化的催化剂。我们改进的程序表明，