Optimization of Coated Blade Spray for Rapid Screening and Quantitation of 105 Veterinary Drugs in Biological Tissue Samples.,Analytical Chemistry

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Optimization of Coated Blade Spray for Rapid Screening and Quantitation of 105 Veterinary Drugs in Biological Tissue Samples.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-31 , DOI: 10.1021/acs.analchem.0c00093
Abir Khaled ₁ , Germán Augusto Gómez-Ríos ₁ , Janusz Pawliszyn ₁

Affiliation

Rapid and efficient determination of contaminants at trace levels in tissue samples has become an unmet need around the globe. Coated blade spray (CBS) extraction/ionization is a technology capable of performing, with a single device, enrichment of analytes present in complex matrices, as well as the direct interface and introduction of said analytes into the mass spectrometer via electrospray ionization. To facilitate the challenging rapid tissue screening, we describe for the first time the use of a very thin layer of biocompatible polyacrylonitrile as a CBS device undercoating to make metal surface biocompatible. This add-on is meant to protect the portion of the uncoated stainless-steel of the blade that is normally exposed to the matrix, consequently becoming susceptible to adhesion of matrix macromolecules, cells, and fat. In addition, we present for the first time the use of CBS in negative ionization mode for quantitative purposes. The optimized CBS workflow allows for rapid and high-throughput screening and quantitation of 105 veterinary drugs in homogenized bovine tissue in both negative and positive ionization mode in one single run using a single CBS device with analysis times as short as 1 min per sample when 96 extractions are simultaneously conducted. While only two internal standards were used for correction, one per ionization mode, excellent accuracy and precision were achieved, with more than 90% of analytes falling within the 70-120% range of their true concentrations and yielding RSD ≤ 25% at three validation levels. The majority of analytes achieved linear correlation coefficients >0.99, and all 105 analytes were able to meet both Canadian and U.S. regulatory levels.

中文翻译：

用于快速筛选和定量生物组织样品中的105种兽药的带涂层叶片喷雾的优化。

快速有效地确定组织样品中痕量水平的污染物已成为全球未满足的需求。涂层叶片喷雾（CBS）提取/电离技术是一种能够通过单个设备进行复杂基质中存在的分析物富集，以及通过电喷雾电离直接将所述分析物引入质谱仪的技术。为了促进具有挑战性的快速组织筛查，我们首次描述了使用非常薄的生物相容性聚丙烯腈作为CBS设备底涂层，以使金属表面具有生物相容性。该附加组件旨在保护叶片的未涂层不锈钢通常暴露于基质的部分，因此变得易于粘附基质大分子，细胞和脂肪。此外，我们首次展示了负离子化模式下CBS的定量应用。优化的CBS工作流程可使用单个CBS设备在一次运行中以负电离和正电离模式快速，高通量筛查和定量均化牛组织中的105种兽药，分析时间短至每个样品1分钟（96）提取同时进行。虽然仅使用两种内标进行校正，但每种电离模式均能实现一种，但仍具有出色的准确性和精密度，其中超过90％的分析物在其真实浓度的70-120％范围内，并且在三项验证中得出RSD≤25％水平。大多数分析物的线性相关系数均> 0.99，并且所有105种分析物均符合加拿大和美国标准

更新日期：2020-04-23

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