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Free Flow Ion Concentration Polarization Focusing (FF-ICPF).
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-30 , DOI: 10.1021/acs.analchem.9b04526
Vasileios A Papadimitriou 1 , Loes I Segerink 1 , Jan C T Eijkel 1
Affiliation  

Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentration polarization focusing (ICPF). The analytes flow continuously through the system via pressure driven flow while they separate and concentrate perpendicularly to the flow by ICPF. We demonstrate free flow ion concentration polarization focusing (FF-ICPF) in two operating modes, namely peak and plateau modes. Additionally, we showed the separation resolution could be improved by the use of an electrophoretic spacer. We report a concentration factor of 10 in human blood plasma in continuous flow at a flow rate of 15 μL min–1.

中文翻译:

自由流动离子浓度极化聚焦(FF-ICPF)。

尽管微流体的固有局限性在于其低样品通量,但微流体中的电动分离技术是一种强大的分析化学工具。在本文中,我们报告了一种电动聚焦方法的自由流动变体,即离子浓度极化聚焦(ICPF)。被分析物通过压力驱动的流动连续流过系统,同时它们被ICPF分离并垂直于流动方向浓缩。我们展示了两种工作模式下的自由流离子浓度极化聚焦(FF-ICPF),即峰模式和平稳模式。此外,我们显示通过使用电泳间隔物可以提高分离分辨率。我们以15μLmin –1的流速连续流动的人体血浆中浓度因子为10 。
更新日期:2020-03-30
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