A Transcriptome Sequencing Study on Genome-Wide Gene Expression Differences of 3D Cultured Chondrocytes in Hydrogel Scaffolds with Different Gel Density.,Macromolecular Bioscience

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A Transcriptome Sequencing Study on Genome-Wide Gene Expression Differences of 3D Cultured Chondrocytes in Hydrogel Scaffolds with Different Gel Density.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-03-18 , DOI: 10.1002/mabi.202000028
Weiyuan Zhang ₁ , Yujun Xia ₁ , Yang Ling ₁ , Wei Yang ₂ , Zuo-Xiang Dong ₃ , Dong-An Wang ₄ , Changjiang Fan _{1,

2}

Affiliation

Hydrogel is considered as a promising cell delivery vehicle in cartilage tissue engineering, whose tunable microenvironments may influence the function and fate of encapsulated chondrocytes. Here, the transcriptomes of chondrocytes that are encapsulated and cultured in hydrogel constructs respectively made of 0.8% and 4% alginate solution are investigated. Differences in chondrocyte transcriptome are detected via RNA‐sequencing from these two cultural conditions. The differentially expressed genes (DEGs) are reflected in extracellular matrix (ECM) secretion, cell cycle, proliferation, cartilage development, and so on. Significantly, the expression of DEGs associated with cartilage ECM and cell proliferation are upregulated in 0.8% constructs; whilst the expressions of DEGs involved in cell cycle and matrix degradation are upregulated in 4% constructs. Moreover, interestingly, the expressions of chondrocyte hypertrophy markers are upregulated in 0.8% constructs; while 4% constructs seemingly favor the long‐term maintenance of chondrocyte phenotype. Taken together, this study confirms on transcriptomic level that gel density affects gene expression and phenotype of the encapsulated chondrocytes; therefore, it may provide guidance for future design and fabrication of cartilage tissue engineering scaffolds.

中文翻译：

不同凝胶密度水凝胶支架中3D培养软骨细胞基因组全基因表达差异的转录组测序研究。

水凝胶在软骨组织工程中被认为是一种有前途的细胞传递载体，其微环境的可调性可能会影响封装的软骨细胞的功能和命运。在这里，研究了分别在0.8％和4％藻酸盐溶液制成的水凝胶构建物中封装和培养的软骨细胞的转录组。通过这两种培养条件下的RNA测序可以检测出软骨细胞转录组的差异。差异表达基因（DEG）反映在细胞外基质（ECM）的分泌，细胞周期，增殖，软骨发育等方面。值得注意的是，与软骨ECM相关的DEGs的表达和细胞增殖在0.8％的构建物中被上调。而参与细胞周期和基质降解的DEGs的表达在4％的构建物中上调。此外，有趣的是，在0.8％的构建体中，软骨细胞肥大标志物的表达上调。而4％的构建体似乎有利于软骨细胞表型的长期维持。两者合计，这项研究在转录组水平上证实凝胶密度影响封装的软骨细胞的基因表达和表型。因此，它可以为软骨组织工程支架的未来设计和制造提供指导。这项研究在转录组水平上证实了凝胶密度会影响封装的软骨细胞的基因表达和表型。因此，它可以为软骨组织工程支架的未来设计和制造提供指导。这项研究在转录组水平上证实了凝胶密度会影响封装的软骨细胞的基因表达和表型。因此，它可以为软骨组织工程支架的未来设计和制造提供指导。

更新日期：2020-03-18

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