A simple, repeatable and inexpensive laboratory practice applied to teach and discuss aquatic metal pollution and oxidative stress detoxification mechanisms through biomarker analyses, as well as important ecotoxicology concepts, is presented herein. It has been implemented in a university in Brazil to both undergraduate and Master's and PhD students, indicating usefulness to all these levels. Students learned to detect metallothionein and reduced glutathione concentrations in biological samples and investigate several variables of interest in biomonitoring assessments. In addition, statistical correlations were used to indicate the potential dual role played by MT in aquatic organisms, allowing for biological inferences regarding both aquatic metal pollution and oxidative stress detoxification mechanisms and maturing of ecotoxicological and biomonitoring concepts discussed and presented both theoretically and integrated to the laboratory findings.

中文翻译：

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金属硫蛋白测定可用于通过基于问题的学习了解水生金属污染和氧化应激解毒机制

本文介绍了一种简单、可重复且廉价的实验室实践，用于通过生物标志物分析以及重要的生态毒理学概念来教授和讨论水生金属污染和氧化应激解毒机制。它已在巴西的一所大学对本科生、硕士和博士生实施，表明对所有这些级别的有用。学生学会了检测生物样品中金属硫蛋白和还原型谷胱甘肽的浓度，并研究生物监测评估中的几个感兴趣的变量。此外，统计相关性被用来表明 MT 在水生生物中发挥的潜在双重作用，