Four species of the Anopheles maculipennis complex have previously been recorded in Sweden. A recent addition to the complex is Anopheles daciae , which is considered to be closely related to, but distinct from Anopheles messeae . The original designation of An. daciae was based on five genetic differences (161, 165, 167, 362 and 382) in the second internal transcribed spacer (ITS) 2 of the ribosomal RNA. Further studies have shown that only two nucleotide differences (362 and 382) robustly separate the species. Thirty‐three An. maculipennis complex mosquitoes were collected in the province of Uppland, Sweden. All were An. daciae but showed double peaks for three variable positions (161, 165 and 167). When cloned, the intra‐individual nucleotide variation was almost exclusively fixed with either TTC or AAT, originally diagnostic for An. messae and An. daciae , respectively. To further investigate the intra‐individual variation, nine An. daciae and 11 An. messeae were collected in southern Sweden and their ITS2 fragments were amplified and sequenced using Illumina MiSeq sequencing (Illumina, Inc., San Diego, CA, USA). For the diagnostic nucleotide 382 no intra‐individual variation could be detected. However, although each An. daciae specimen carried several ITS2 sequence variants for the four other nucleotides, there was no intra‐individual variation in the An. messeae specimens.

中文翻译：

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瑞典两种同伴疟疾蚊子（大按蚊和信使按蚊）ITS2区的单核苷酸多态性分析。

瑞典以前曾记录过四种Anopheles maculipennis复合体。最近除了复杂的是按蚊daciae，这被认为是密切相关的，但是从不同的按蚊messeae。An的原始名称。daciae基于核糖体RNA的第二个内部转录间隔区（ITS）2中的五个遗传差异（161、165、167、362和382）。进一步的研究表明，只有两个核苷酸差异（362和382）可以牢固地分隔物种。三十三的。Maculipennis复杂的蚊子在瑞典的Uppland省收集。都是安。daciae但在三个可变位置（161、165和167）显示双峰。克隆后，个体内部核苷酸变异几乎完全由最初诊断为An的TTC或AAT固定。梅沙和安。daciae。为了进一步研究个体内变异，九一。daciae和11 An。messeae收集在瑞典南部和他们的ITS2片段进行扩增和测序利用Illumina MiSeq测序（Illumina公司，圣地亚哥，CA，USA）。对于诊断核苷酸382，无法检测到个体内部变异。但是，虽然每一个。daciae标本带有其他四个核苷酸的几个ITS2序列变异体，An中没有个体内变异。信使标本。