Myeloid differentiation factor 88 (Myd88) plays an important role in both innate and adaptive immune response. In this study, the full-length complementary DNA (cDNA) of myd88 from Misgurnus anguillicaudatus was characterized. The myd88 cDNA is 1333 bp in length and contains an 855 bp open reading frame encoding a predicted protein of 284 amino acids. The predicted protein possesses typical Myd88 domain structural features including a death domain in the N-terminus, and box 1, 2, and 3 motifs of the Toll/IL-1 receptor domain in the C-terminus. Quantitative real-time PCR (qRT-PCR) revealed that myd88 messenger RNA (mRNA) was ubiquitously expressed in all examined tissues, especially highly in brain, kidney, blood, intestines and liver. qRT-PCR and western blotting were used to determine the mRNA and protein levels of Myd88 after Aeromonas veronii challenge, respectively. The Myd88 was remarkably upregulated in response to infection of A. veronii. These results suggested that Myd88 may play a vital role during the immune response of M. anguillicaudatus against bacterial infection.

中文翻译：

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东方风鱼（Misgurnus anguillicaudatus）myd88响应细菌攻击的分子克隆及表达分析

髓样分化因子 88 (Myd88) 在先天性和适应性免疫反应中都起着重要作用。在这项研究中，对来自 Misgurnus anguillicaudatus 的 myd88 的全长互补 DNA (cDNA) 进行了表征。myd88 cDNA 长度为 1333 bp，包含一个 855 bp 的开放阅读框，编码 284 个氨基酸的预测蛋白质。预测的蛋白质具有典型的 Myd88 域结构特征，包括 N 端的死亡域和 C 端的 Toll/IL-1 受体域的框 1、2 和 3 基序。定量实时 PCR (qRT-PCR) 显示 myd88 信使 RNA (mRNA) 在所有检查的组织中普遍表达，尤其是在脑、肾、血液、肠和肝脏中。qRT-PCR 和蛋白质印迹分别用于确定维罗单胞菌攻击后 Myd88 的 mRNA 和蛋白质水平。Myd88 在响应 A. veronii 感染时显着上调。这些结果表明，Myd88 可能在 M. anguillicaudatus 对细菌感染的免疫反应中起重要作用。