A 56‐day feeding trial was conducted to investigate the effects of replacing fish meal with cottonseed protein concentrate (CPC) (free gossypol < 7.9 mg/kg) in the diets on the growth, intestinal microflora, haematological and antioxidant indices of juvenile golden pompano (Trachinotus ovatus). Six diets were designed: fishmeal diets (FM) which contained 340 g/kg fishmeal, as well as five CPC diets, each with differing CPC concentrations (120, 240, 360, 480 and 600 g/kg) to replace the fish meal. The weight gain rate (WGR) and specific growth rate (SGR) showed no significant difference among groups (p > .05) with the dietary CPC level ranged from 0 to 360 g/kg. Serum cholesterol (CHO) of C36 and triglyceride (TG) levels of C36 and C12 were significantly higher than the FM (p < .05). Total protein (TP) levels of C12 were significantly lower than the FM (p < .05). Among the treatments, C36 had higher glutathione peroxidase (GSH‐PX) and total superoxide dismutase (T‐SOD) than FM (p < .05). From the data analysis of 16s sequencing, with increasing CPC concentration, the proportion of harmful microbial taxa (Proteobacteria and Vibrio) increased. The results of this study support that CPC products are acceptable in practical diets for golden pompano. And the optimal dietary CPC replacement of golden pompano was estimated to be 259.3 g/kg.

中文翻译：

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用棉籽蛋白浓缩物代替鱼粉对少年金po（Trachinotus ovatus）的生长，肠道菌群，血液学和抗氧化指标的影响

进行了为期56天的喂养试验，以研究日粮中用棉籽蛋白浓缩物（CPC）（游离棉酚<7.9 mg / kg）代替鱼粉对金ju鱼的生长，肠道菌群，血液学和抗氧化指标的影响（Trachnotus ovatus）。设计了六种饮食：包含340克/千克鱼粉的鱼粉饮食（FM），以及五种CPC饮食，每种饮食具有不同的CPC浓度（120、240、360、480和600 g / kg）来代替鱼粉。饮食中CPC水平在0至360 g / kg之间，体重增加率（WGR）和比生长率（SGR）在各组之间无显着差异（p>  .05）。C36的血清胆固醇（CHO）和C36和C12的甘油三酸酯（TG）水平显着高于FM（p  <.05）。C12的总蛋白（TP）水平显着低于FM（p  <.05）。在这些治疗中，C36的谷胱甘肽过氧化物酶（GSH-PX）和总超氧化物歧化酶（T-SOD）高于FM（p <  .05）。从16s测序数据分析来看，随着CPC浓度的增加，有害微生物分类群（Proteobacteria和Vibrio）的比例增加。这项研究的结果支持CPC产品在金po鱼的实际饮食中是可以接受的。估计金CPC鱼的最佳膳食CPC替代为259.3 g / kg。