In this manuscript, three iso‐nitrogenous and iso‐lipidic diets containing 50, 150 and 250 g/kg of fishmeal (FM) were fed white shrimp Litopenaeus vannamei for 8 weeks. At the end of feeding, survival and percentage weight gain of shrimp were examined. Hepatopancreas and haemolymph were sampled, activities of superoxide dismutase (SOD) and catalase (CAT), nitric oxide (NO), glutathione (GSH) and malondialdehyde (MDA) levels in hepatopancreas, activities of SOD, CAT, acid phosphatase (ACP) and alkaline phosphatase (AKP), and NO, GSH, MDA in haemolymph were examined. Haemolymph was further subjected to GC‐MS analysis. Results indicated that no significant differences in survival rate and percentage weight gain were observed among three treatments. SOD activity and GSH levels in hepatopancreas, and CAT activity, AKP activity, GSH and MDA levels in haemolymph were significantly lower in shrimp fed 50 g/kg FM diet. GC‐MS analysis of haemolymph indicated that 81 metabolites were significantly altered in the three groups. Furthermore, 8 metabolism pathways were significantly influenced by dietary FM levels. In conclusion, dietary administration of 50 g/kg FM resulted in inhibition of antioxidant ability, and disordered the energy metabolism, one‐carbon metabolism and unsaturated fatty acid metabolism in shrimp.

中文翻译：

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膳食鱼粉水平影响南美白对虾幼虾的抗氧化能力和代谢组学特征

在这份手稿中，将含有 50、150 和 250 g/kg 鱼粉 (FM) 的三种异氮和异脂日粮饲喂南美白对虾白虾 8 周。在喂食结束时，检查虾的存活率和增重百分比。对肝胰腺和血淋巴取样，超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 的活性，肝胰腺中的一氧化氮 (NO)、谷胱甘肽 (GSH) 和丙二醛 (MDA) 水平，SOD、CAT、酸性磷酸酶 (ACP) 和检测血淋巴中的碱性磷酸酶（AKP）和NO、GSH、MDA。血淋巴进一步进行GC-MS分析。结果表明，在三种处理中未观察到存活率和体重增加百分比的显着差异。肝胰腺中的 SOD 活性和 GSH 水平，以及 CAT 活性、AKP 活性、喂食 50 g/kg FM 饲料的虾的血淋巴中 GSH 和 MDA 水平显着降低。血淋巴的 GC-MS 分析表明，三组中有 81 种代谢物发生了显着改变。此外，8 种代谢途径受到膳食 FM 水平的显着影响。综上所述，日粮添加 50 g/kg FM 会抑制虾的抗氧化能力，并扰乱虾的能量代谢、一碳代谢和不饱和脂肪酸代谢。