Liver cells were isolated from 6 fish fed a diet containing 12.1 g methionine/kg, 11.02 mg vitamin B6/kg, 0.20 mg vitamin B12/kg and 7.80 mg folate/kg (named high‐1C diet). These cells were compared to liver cells isolated from 6 fish fed a diet containing 6.7 g methionine/kg, 7.01 g vitamin B6/kg, 0.15 mg vitamin B12/kg and 2.60 mg folate/kg (named low‐1C diet). Isolated cells were plated on 6‐well plates in Leibovitz medium and treated with 10 mM metformin, 10 mM metformin for 24 hr followed by 0.4 mM oleic acid (OA) for 24 hr or only 0.4 mM OA for 24 hr. The cells were compared to untreated controls added only the medium. All cells were harvested 48 hr after being plated. Cells isolated from Atlantic salmon fed low‐1C diets showed higher gene expression of MGAT‐2 (p < .0001), CPT‐1 (p = .028), FAS (p = .0006), LXR (p = .020), ACC (p = .032) and MnSOD (p < .0001). The low‐ or high‐1C diets fed prior to cell isolation had no effect on gene expression of ApoB100, PPARa, CD36, SREBP‐2 or Bcl‐2. Metformin treatment increased the expression of the anti‐apoptotic protein Bcl‐2 (p = .0001) indicating an anti‐apoptotic effect. Metformin generally increased the expression of genes associated with lipid oxidation and transport, but decreased the expression of genes associated with cholesterol metabolism confirming our earlier results using this model.

中文翻译：

---

细胞分离前饲喂的1C饮食水平会影响从大西洋鲑鱼（Salmo salar）分离的原代肝细胞的脂质代谢

从6条喂食日粮的鱼中分离出肝细胞，日粮中含有12.1 g蛋氨酸/kg、11.02 mg维生素B6 / kg，0.20 mg维生素B12 / kg和7.80 mg叶酸/ kg（称为高1C饮食）。将这些细胞与从6条鱼中分离得到的肝细胞进行比较，这些鱼的饮食分别为6.7 g蛋氨酸/kg、7.01 g维生素B6 / kg，0.15 mg维生素B12 / kg和2.60 mg叶酸/ kg（称为低-1C饮食）。将分离的细胞接种在Leibovitz培养基中的6孔板上，用10 mM二甲双胍，10 mM二甲双胍处理24小时，然后用0.4 mM油酸（OA）处理24小时或仅使用0.4 mM OA处理24小时。将细胞与仅添加培养基的未处理对照进行比较。铺板后48小时收获所有细胞。从大西洋鲑饲喂低1C饮食的细胞显示出MGAT-2（p  <.0001），CPT-1（p = .028），FAS（p  = .0006），LXR（p  = .020），ACC（p  = .032）和MnSOD（p  <.0001）。在分离细胞之前进食的低或高1C饮食对ApoB100，PPARa，CD36，SREBP-2或Bcl-2的基因表达没有影响。二甲双胍治疗可增加抗凋亡蛋白Bcl-2的表达（p  = .0001），表明具有抗凋亡作用。二甲双胍通常会增加与脂质氧化和转运相关的基因表达，但会降低与胆固醇代谢相关的基因表达，这证实了我们使用该模型的较早结果。