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The effect of dietary folic acid on flesh quality and muscle antioxidant status referring to Nrf2 signalling pathway in young grass carp ( Ctenopharyngodon idella )
Aquaculture Nutrition ( IF 3.5 ) Pub Date : 2020-02-16 , DOI: 10.1111/anu.13023
Lei Shi 1 , Xiao‐Qiu Zhou 1 , Wei‐Dan Jiang 1, 2, 3 , Pei Wu 1, 2, 3 , Yang Liu 1, 2, 3 , Jun Jiang 1, 2, 3 , Sheng‐Yao Kuang 4 , Ling Tang 4 , Lin Feng 1, 2, 3
Affiliation  

To investigate the effect of dietary folic acid on fish flesh quality, muscle antioxidant status and the potential mechanism, young grass carp (Ctenopharyngodon idella) were fed diets containing 0.10–3.12 mg/kg diet of folic acid for 8 weeks. The results showed that optimum folic acid increased muscle contents of protein, lipid, pH, shear force, amino acids, unsaturated fatty acids, hydroxyproline and glutathione, whereas contents of moisture, cooking loss, lactate and saturated fatty acids and cathepsin L activity showed an opposite trend (p < .05). Moreover, optimum folic acid elevated antioxidant enzyme activities and mRNA levels, as well as NF‐E2‐related factor 2 and casein kinase 2 mRNA levels (p < .05). However, optimum folic acid decreased reactive oxygen species (ROS) content and Kelch‐like ECH‐associated protein 1a (Keap1a) and Keap1b mRNA levels (p < .05). Interestingly, excess folic acid induced negative effects on above‐mentioned parameters (p < .05). Summarily, this study indicated that optimum folic acid improved fish flesh quality and muscle antioxidant system associated with Nrf2‐Keap1 pathway. Based on muscle cathepsin L activity and ROS content, the folic acid requirements for young grass carp were 1.87 and 1.80 mg/kg diet, respectively, regarding to the flesh quality and antioxidant status.

中文翻译:

日粮叶酸对草鱼幼鱼肉质和肌肉抗氧化状态的影响与Nrf2信号通路有关

为了研究日粮叶酸对鱼肉质量、肌肉抗氧化状态和潜在机制的影响,用含有 0.10-3.12 mg/kg 叶酸的日粮喂养幼草鱼(Ctenopharyngodon idella)8 周。结果表明,最佳叶酸增加了肌肉中蛋白质、脂质、pH、剪切力、氨基酸、不饱和脂肪酸、羟脯氨酸和谷胱甘肽的含量,而水分、蒸煮损失、乳酸和饱和脂肪酸的含量以及组织蛋白酶 L 活性显示出相反的趋势(p < .05)。此外,最佳叶酸可提高抗氧化酶活性和 mRNA 水平,以及 NF-E2 相关因子 2 和酪蛋白激酶 2 mRNA 水平(p < .05)。然而,最佳叶酸可降低活性氧 (ROS) 含量和 Kelch 样 ECH 相关蛋白 1a (Keap1a) 和 Keap1b mRNA 水平 (p < .05)。有趣的是,过量的叶酸会对上述参数产生负面影响(p < .05)。总之,这项研究表明,最佳叶酸可改善与 Nrf2-Keap1 通路相关的鱼肉质量和肌肉抗氧化系统。根据肌肉组织蛋白酶 L 活性和 ROS 含量,考虑到肉质和抗氧化状态,草鱼幼鱼的叶酸需求量分别为 1.87 和 1.80 mg/kg 日粮。该研究表明,最佳叶酸可改善与 Nrf2-Keap1 通路相关的鱼肉质量和肌肉抗氧化系统。根据肌肉组织蛋白酶 L 活性和 ROS 含量,考虑到肉质和抗氧化状态,草鱼幼鱼的叶酸需求量分别为 1.87 和 1.80 mg/kg 日粮。该研究表明,最佳叶酸可改善与 Nrf2-Keap1 通路相关的鱼肉质量和肌肉抗氧化系统。根据肌肉组织蛋白酶 L 活性和 ROS 含量,考虑到肉质和抗氧化状态,草鱼幼鱼的叶酸需求量分别为 1.87 和 1.80 mg/kg 日粮。
更新日期:2020-02-16
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