A 45‐day feeding trial was conducted to evaluate the utilization of fermented plant protein mix (FPPM) as a fishmeal substitute in the diet of Penaeus vannamei. Six isonitrogenous and isolipidic diets were formulated by substituting fishmeal at 0% (control), 50%, 60%, 70%, 80% and 90% (FR‐0, FR‐50, FR‐60, FR‐70, FR‐80 and FR‐90, respectively) using FPPM. Shrimps fed with diets FR‐50, FR‐60 and FR‐70 had no significant difference in weight gain (173.22%–178.07%) with the control group (179.50%). However, the broken‐line regression indicated that 70.8% fishmeal could be substituted by FPPM. The trend of digestibility was reflected in growth performance. Survival had no significant difference among the treatments. Fishmeal substitution had significant (p < .05) variations in protease and amylase activity, but not in lipase. Except lipid, other body composition was not affected due to dietary change. A decrease (p < .05) in haemolymph total protein, cholesterol and triglycerides was observed to increase in fishmeal substitution. A higher fishmeal substitution (FR‐90) had significantly (p < .05) lower total haemocyte count and the reverse was true for phenoloxidase. Results conclude that a combination of fermented ingredients would be more effective rather using alone in shrimp feed, with a great potential in reducing the pressure on fishmeal.

中文翻译：

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黑曲霉发酵植物蛋白混合物作为南美白对虾饮食中鱼粉的潜在替代品（Boone，1931）

进行了一项为期 45 天的饲养试验，以评估发酵植物蛋白混合物 (FPPM) 在南美白对虾饲料中作为鱼粉替代品的利用。通过在 0%（对照）、50%、60%、70%、80% 和 90%（FR-0、FR-50、FR-60、FR-70、FR- 80 和 FR-90，分别）使用 FPPM。饲喂 FR-50、FR-60 和 FR-70 日粮的虾与对照组 (179.50%) 的体重增加 (173.22%–178.07%) 没有显着差异。然而，折线回归表明 70.8% 的鱼粉可以被 FPPM 替代。消化率的趋势反映在生长性能上。各处理间存活率无显着差异。鱼粉替代品在蛋白酶和淀粉酶活性方面有显着变化（p < .05），但在脂肪酶方面没有变化。除了脂质，其他身体成分未因饮食改变而受到影响。观察到血淋巴总蛋白、胆固醇和甘油三酯的减少（p < .05）会增加鱼粉替代品。较高的鱼粉替代量 (FR-90) 显着 (p < .05) 降低总血细胞计数，而酚氧化酶则相反。结果表明，发酵成分的组合比单独用于虾饲料更有效，在降低鱼粉压力方面具有巨大潜力。