This study was carried out to investigate the effects of dietary pyridoxine (PN) on the antioxidant status, apoptosis, intercellular integrity and immune function of head kidney and spleen in young grass carp (Ctenopharyngodon idella). Results showed that compared with the optimal PN level, (a) PN deficiency decreased antioxidant enzyme activities and down‐regulated the mRNA levels of antioxidant enzymes, NF‐E2‐related factor 2 (Nrf2), myeloid cell leukaemia‐1 (Mcl‐1) and tight junction proteins, whereas it increased the contents of reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC), and up‐regulated the mRNA levels of cysteinyl aspartic acid‐protease (caspase), Fas ligand (FasL), p38 mitogen‐activated protein kinase (p38MAPK) and myosin light chain kinase (MLCK) in the head kidney and spleen of fish (p < .05). (b) PN deficiency decreased lysozyme (LZ) and acid phosphatase (ACP) activities, and complement contents, and down‐regulated the mRNA levels of antibacterial peptides, anti‐inflammatory cytokines, inhibitor of κBα (IκBα) and target of rapamycin (TOR), whereas it up‐regulated the mRNA levels of pro‐inflammatory cytokines and nuclear factor kappa B p65 (NF‐κBp65) in the head kidney and spleen of fish (p < .05). (c) Dietary PN requirements of grass carp based on the malondialdehyde content and lysozyme activity were estimated as 4.97 and 4.99 mg/kg diet, respectively.

中文翻译：

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饲喂不同水平吡哆醇对草鱼(Ctenopharyngodon idella)头肾和脾的抗氧化状态、细胞凋亡、细胞间完整性和免疫功能的影响

本研究旨在研究日粮吡哆醇 (PN) 对草鱼 (Ctenopharyngodon idella) 头肾和脾脏的抗氧化状态、细胞凋亡、细胞间完整性和免疫功能的影响。结果表明，与最佳 PN 水平相比，(a) PN 缺乏降低了抗氧化酶活性并下调了抗氧化酶、NF-E2 相关因子 2 (Nrf2)、髓细胞白血病-1 (Mcl-1) 的 mRNA 水平。 ) 和紧密连接蛋白，而它增加了活性氧 (ROS)、丙二醛 (MDA) 和蛋白羰基 (PC) 的含量，并上调了半胱氨酰天冬氨酸蛋白酶 (caspase)、Fas 配体的 mRNA 水平（ FasL)、p38 丝裂原活化蛋白激酶 (p38MAPK) 和鱼头肾和脾脏中的肌球蛋白轻链激酶 (MLCK) (p < .05)。(b) PN 缺乏会降低溶菌酶 (LZ) 和酸性磷酸酶 (ACP) 活性以及补体含量，并下调抗菌肽、抗炎细胞因子、κBα 抑制剂 (IκBα) 和雷帕霉素靶标 (TOR) 的 mRNA 水平)，而它上调了鱼的头肾和脾脏中促炎细胞因子和核因子κB p65 (NF-κBp65) 的 mRNA 水平（p < .05）。(c) 基于丙二醛含量和溶菌酶活性的草鱼膳食 PN 需求分别估计为 4.97 和 4.99 mg/kg 饲料。而它上调了鱼头肾和脾脏中促炎细胞因子和核因子κB p65（NF-κBp65）的mRNA水平（p < .05）。(c) 基于丙二醛含量和溶菌酶活性的草鱼膳食 PN 需求分别估计为 4.97 和 4.99 mg/kg 饲料。而它上调了鱼头肾和脾脏中促炎细胞因子和核因子κB p65（NF-κBp65）的mRNA水平（p < .05）。(c) 基于丙二醛含量和溶菌酶活性的草鱼膳食 PN 需求量分别估计为 4.97 和 4.99 mg/kg 饲料。