A 12‐week feeding trial was conducted to investigate the effects of two dietary probiotics; Bacillus subtilis KCTC 2217 or Bacillus licheniformis KCCM 11775 with two prebiotics; mannan oligosaccharide (MOS) or fructooligosaccharide (FOS) in Japanese eel. Fish averaging 12.8 ± 0.47 g (mean ± SD) were randomly distributed into five treatments with triplicate tanks. A basal control diet (CON) and four synbiotic diets supplementing B. subtilis + MOS (BSM), B. subtilis + FOS (BSF), B. licheniformis + MOS (BLM), and B. licheniformis + FOS (BLF). Weight gain and specific growth rate of fish fed all synbiotic diets were higher than those of fish fed CON. Immune‐related gene expression of heat shock protein 70 and immunoglobulin M of fish fed BSF and BLM were significantly higher than those of fish fed CON. Fish fed BSF and BLM had significantly higher intestinal villi length than those of fish fed BLF and CON (p < .05). Disease resistance against Aeromonas hydrophila of fish fed all synbiotic diets were significantly higher than those of fish fed CON (p < .05). Therefore, these results indicated that dietary B. subtilis with FOS (BSF) and B. licheniformis with MOS (BLM) could have beneficial effects on intestinal morphology, and immune‐related gene expression in Japanese eel.

中文翻译：

---

两种膳食益生菌（枯草芽孢杆菌或地衣芽孢杆菌）与两种益生元（甘露聚糖或低聚果糖）对日本鳗鲡的影响

进行了为期 12 周的喂养试验，以研究两种膳食益生菌的效果；枯草芽孢杆菌 KCTC 2217 或地衣芽孢杆菌 KCCM 11775 与两种益生元；日本鳗鱼中的甘露寡糖 (MOS) 或低聚果糖 (FOS)。平均 12.8 ± 0.47 g（平均值 ± SD）的鱼被随机分配到五个处理中，三个处理池。一种基础对照饮食 (CON) 和四种补充 B. subtilis + MOS (BSM)、B. subtilis + FOS (BSF)、B. licheniformis + MOS (BLM) 和 B. licheniformis + FOS (BLF) 的合生元饮食。饲喂所有合生元饲料的鱼的增重和比生长率均高于饲喂 CON 的鱼。饲喂 BSF 和 BLM 的鱼的热休克蛋白 70 和免疫球蛋白 M 的免疫相关基因表达显着高于饲喂 CON 的鱼。饲喂 BSF 和 BLM 的鱼的肠绒毛长度明显高于饲喂 BLF 和 CON 的鱼（p < .05）。饲喂所有合生元饲料的鱼对嗜水气单胞菌的抗病性显着高于饲喂 CON 的鱼（p < .05）。因此，这些结果表明日粮中含有 FOS (BSF) 的枯草芽孢杆菌和含有 MOS 的地衣芽孢杆菌 (BLM) 可能对日本鳗鱼的肠道形态和免疫相关基因表达产生有益影响。