BACKGROUND In several species, paraoxonase-1 (PON-1) decreases during inflammation, because of the presence of oxidative stress; its measurement recently has been validated in horses, but its role as a clinical biomarker is unknown. OBJECTIVES To evaluate sensitivity, specificity and likelihood ratio of PON-1 activity to identify systemic inflammatory response syndrome (SIRS)-positive horses or horses with a poor prognosis. ANIMALS One hundred seventy-two blood samples from 58 sick horses from 3 different veterinary hospitals. METHODS In a cross-sectional study, PON-1 activity was measured upon admission and at 24-hour intervals until discharge or death, and results were analyzed based on SIRS status and outcome. RESULTS No statistically significant difference was found in median PON-1 activity between SIRS and non-SIRS cases or between survivors and non-survivors except for mares, in which PON-1 activity was significantly lower in SIRS-positive horses (P = .05). The sensitivity of PON-1 activity in identifying horses with SIRS or negative outcome was low (0.0%-46.2% depending on the examined group) but its specificity was high (87.0%-100.0%). However, when PON-1 is low, the likelihood of death is 2.40-3.89 times higher than the likelihood of survival. Repeated measurement of PON-1 after treatment does not predict outcome. CONCLUSIONS AND CLINICAL IMPORTANCE Evaluation of PON-1 activity in horses with inflammation might be advisable in the future, but only low activity at admission may be relevant in predicting SIRS or negative outcome.

中文翻译：

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对氧磷酶-1活性评估作为马和驹中的诊断和预后标志物。

背景技术在几种物种中，由于氧化应激的存在，对氧磷酶-1（PON-1）会在炎症过程中减少。它的测量值最近已在马匹中得到验证，但其作为临床生物标记物的作用尚不清楚。目的评估PON-1活性的敏感性，特异性和似然比，以鉴定系统性炎症反应综合征（SIRS）阳性的马或预后不良的马。动物从3家不同的兽医医院的58匹病马中提取了172个血液样本。方法在一项横断面研究中，在入院时和间隔24小时直至出院或死亡之前测量PON-1的活性，并根据SIRS的状态和结果分析结果。结果SIRS与非SIRS病例之间或存活者与非存活者之间的PON-1活性中值无统计学差异，除了母马，其中SIRS阳性马的PON-1活性明显降低（P = 0.05） ）。PON-1活性在识别SIRS或阴性结果的马中的敏感性较低（根据所检查的组为0.0％-46.2％），但特异性较高（87.0％-100.0％）。但是，当PON-1较低时，死亡的可能性是存活可能性的2.40-3.89倍。治疗后重复测量PON-1不能预测结果。结论和临床意义评估炎症性马匹中PON-1的活性可能在未来是可取的，但是入院时低活性可能与SIRS预测或阴性结果有关。