Functional analyses of small secreted cysteine-rich proteins identified candidate effectors in Verticillium dahliae.,Molecular Plant Pathology

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Functional analyses of small secreted cysteine-rich proteins identified candidate effectors in Verticillium dahliae.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-03-10 , DOI: 10.1111/mpp.12921
Dan Wang _{1,

2} , Li Tian ₃ , Dan-Dan Zhang _{1,

2,

4} , Jian Song _{1,

2} , Shuang-Shuang Song ₃ , Chun-Mei Yin ₁ , Lei Zhou _{1,

2,

4} , Yan Liu ₃ , Bao-Li Wang ₁ , Zhi-Qiang Kong _{1,

2} , Steven J Klosterman ₅ , Jun-Jiao Li _{1,

2} , Jie Wang ₁ , Ting-Gang Li ₁ , Sabiu Adamu _{1,

2} , Krishna V Subbarao ₆ , Jie-Yin Chen _{1,

2,

4} , Xiao-Feng Dai _{1,

2,

4}

Affiliation

Secreted small cysteine‐rich proteins (SCPs) play a critical role in modulating host immunity in plant–pathogen interactions. Bioinformatic analyses showed that the fungal pathogen Verticillium dahliae encodes more than 100 VdSCPs, but their roles in host–pathogen interactions have not been fully characterized. Transient expression of 123 VdSCP‐encoding genes in Nicotiana benthamiana identified three candidate genes involved in host–pathogen interactions. The expression of these three proteins, VdSCP27, VdSCP113, and VdSCP126, in N. benthamiana resulted in cell death accompanied by a reactive oxygen species burst, callose deposition, and induction of defence genes. The three VdSCPs mainly localized to the periphery of the cell. BAK1 and SOBIR1 (associated with receptor‐like protein) were required for the immunity triggered by these three VdSCPs in N. benthamiana. Site‐directed mutagenesis showed that cysteine residues that form disulphide bonds are essential for the functioning of VdSCP126, but not VdSCP27 and VdSCP113. VdSCP27, VdSCP113, and VdSCP126 individually are not essential for V. dahliae infection of N. benthamiana and Gossypium hirsutum, although there was a significant reduction of virulence on N. benthamiana and G. hirsutum when inoculated with the VdSCP27/VdSCP126 double deletion strain. These results illustrate that the SCPs play a critical role in the V. dahliae–plant interaction via an intrinsic virulence function and suppress immunity following infection.

中文翻译：

对富含半胱氨酸的小分泌蛋白的功能分析确定了大丽黄萎病菌中的候选效应子。

分泌的富含半胱氨酸的小蛋白（SCP）在植物与病原体相互作用中调节宿主免疫方面发挥着关键作用。生物信息学分析表明，真菌病原体大丽黄萎病编码超过 100 个 VdSCP，但它们在宿主与病原体相互作用中的作用尚未完全表征。本氏烟草中 123 个 VdSCP 编码基因的瞬时表达鉴定出三个参与宿主-病原体相互作用的候选基因。本塞姆氏烟草中这三种蛋白（VdSCP27、VdSCP113 和 VdSCP126）的表达导致细胞死亡，并伴有活性氧爆发、胼胝质沉积和防御基因的诱导。三个VdSCP主要位于细胞的外围。BAK1 和 SOBIR1（与受体样蛋白相关）是本塞姆氏烟草中这三种 VdSCP 触发的免疫所必需的。定点诱变表明，形成二硫键的半胱氨酸残基对于 VdSCP126 的功能至关重要，但对 VdSCP27 和 VdSCP113 则不然。VdSCP27、VdSCP113和VdSCP126单独对于本塞姆氏烟草和陆地棉的大丽弧菌感染不是必需的，尽管当接种VdSCP27 / VdSCP126双缺失菌株时，本塞姆氏烟草和陆地棉的毒力显着降低。这些结果表明，SCP通过内在毒力功能在大丽葡萄球菌与植物相互作用中发挥着关键作用，并抑制感染后的免疫力。

更新日期：2020-03-10

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