The artificial cultivation of moss biocrusts can accelerate the recovery of degraded arid lands and is closely related to moss productivity. Understanding the properties of inoculation materials on the regenerative capacity of mosses, in particular the effect of time limits on storage, has the potential to benefit the cultivation of artificial moss biocrusts. We investigated the vegetative regeneration and physiological characteristics of three desiccation‐tolerant mosses (Barbula unguiculata, Didymodon vinealis, and Didymodon tectorum) upon rehydration after periods of desiccation storage for 40, 89, 127, and 197 days. Regenerative capacity, represented by gametophyte vigor index, decreased with increased storage time. The greatest change in vegetative regeneration among storage times was observed in B. unguiculata, where the gametophyte vigor index decreased by 95.74% after 197 days of storage. Over the same period, there were smaller decreases in gametophyte vigor index of D. vinealis and D. tectorum of 42.17% and 13.30%, respectively. Malondialdehyde and soluble sugar increased with longer periods of storage time, while soluble protein content first increased, then decreased. Oxidation levels are important factors influencing the recovery of desiccation‐tolerant mosses. All three moss species regenerated after 197 days of storage, but regenerative capacity is dependent on species. For land managers, biocrust restoration can be facilitated by careful screening of suitable moss species, based on their capacity to regenerate new growth after extended periods of storage and selection of species based on variations in physiological characteristics.

中文翻译：

---

贮藏时间对黄土高原耐旱苔藓生理特性和营养更新的影响

人工种植苔藓生物结皮可以促进退化干旱土地的恢复，并且与苔藓生产力密切相关。了解接种材料对苔藓再生能力的特性，特别是时间限制对储存的影响，有可能有益于人工苔藓生物结壳的种植。我们调查了三种耐旱苔藓（Barbula unguiculata ， Didymodon vinealis和Didymodon tectorum）的营养更新和生理特性。），在干燥期40、89、127和197天后重新水化。以配子体活力指数表示的再生能力随储存时间的增加而降低。在贮藏时间中，无性芽孢杆菌的营养再生发生了最大的变化，在贮藏197天后，配子体活力指数下降了95.74％。在同一时期，葡萄藤和胸腺的配子体活力指数下降幅度较小。分别为42.17％和13.30％。丙二醛和可溶性糖随着储存时间的延长而增加，而可溶性蛋白质含量先增加，然后减少。氧化水平是影响耐干燥苔藓恢复的重要因素。三种青苔物种在储存197天后都会再生，但是其再生能力取决于物种。对于土地管理者来说，基于适当的苔藓物种的长期筛选和基于生理特征变化的物种再生能力，可以通过仔细筛选合适的苔藓物种来促进生物结皮的恢复。