Abstract The title compound 2-[2-(2-fluorobenzylidene)hydrazinyl]-4-(1-methyl-1H-indol-3-yl) thieno[3,2-d]pyrimidine (C22H16FN5S) was prepared and its structure was confirmed by IR, 1H NMR, MS, elemental analyses and X-ray diffraction. The crystal of the title compound belongs to the monoclinic system, space group P21/c with a = 14.4546(17) Å, b = 17.0895(19) Å, c = 17.9621(15) Å, α = 90°, β = 122.717(6), γ = 90°, V = 3733.1(7) Å3, Z = 4, and R = 0.0412 for 4816 observed reflections with I > 2σ(I). In addition, the compound possesses distinct effective inhibition on the proliferation of HT-29, A549 and MKN45 cell lines.

中文翻译：

---

2-[2-(2-氟亚苄基)肼基]-4-(1-甲基-1H-吲哚-3-基)噻吩并[3,2-d]嘧啶的合成、晶体和抗增殖活性

摘要 制备了标题化合物2-[2-(2-氟亚苄基)肼基]-4-(1-甲基-1H-吲哚-3-基)噻吩并[3,2-d]嘧啶(C22H16FN5S)，其结构为经 IR、1H NMR、MS、元素分析和 X 射线衍射证实。标题化合物的晶体属于单斜晶系，空间群P21/c，a = 14.4546(17) Å, b = 17.0895(19) Å, c = 17.9621(15) Å, α = 90°, β = 122.717 (6), γ = 90°，V = 3733.1(7) Å3，Z = 4，对于 4816 次观测到的反射，I > 2σ(I)，R = 0.0412。此外，该化合物对 HT-29、A549 和 MKN45 细胞系的增殖具有明显的有效抑制作用。