ABSTRACT Herein, we present a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method for the determination of semicarbazide (SEM) using a molecularly imprinted polymer (MIP) film as the bionic antibody. The best monomer (methacrylic acid) and molar ratio of monomer to template (4:1) were determined with the aid of computational simulation methods based on density functional theory (DFT). The MIP film was characterised by scanning electron microscopy and adsorption experiments. The results showed that the imprinted film was successfully synthesised with good adsorption performance. Under optimal conditions, the calibration curve was linear in the range of 1.0–1.0×104 ng/mL (R 2 = 0.9863). The detection limit (IC15) and sensitivity (IC50) were 1.0 and 85.7 ng/mL, respectively. This BELISA method was further applied to the analysis of SEM in food samples; recoveries ranged from 86.1% to 90.8%. These observations indicated that the developed method could be adapted for SEM quantification in food.

中文翻译：

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基于分子印迹技术的氨基脲仿生酶联免疫吸附试验的研制

摘要在此，我们提出了一种使用分子印迹聚合物 (MIP) 膜作为仿生抗体测定氨基脲 (SEM) 的直接竞争仿生酶联免疫吸附测定 (BELISA) 方法。借助基于密度泛函理论（DFT）的计算模拟方法，确定了最佳单体（甲基丙烯酸）和单体与模板的摩尔比（4:1）。通过扫描电子显微镜和吸附实验对 MIP 膜进行了表征。结果表明，印迹薄膜合成成功，具有良好的吸附性能。在最佳条件下，校准曲线在 1.0–1.0×104 ng/mL 范围内呈线性（R 2 = 0.9863）。检测限 (IC15) 和灵敏度 (IC50) 分别为 1.0 和 85.7 ng/mL。该BELISA方法进一步应用于食品样品中SEM的分析；回收率从 86.1% 到 90.8% 不等。这些观察结果表明，所开发的方法可适用于食品中的 SEM 定量。