ABSTRACT Aminophylline (AMP), a bronchodilator drug, is a modified form of theophylline (the pharmacological active ingredient). AMP is often given to treat respiratory diseases, including bronchial asthma, blocked emphysema, panting bronchitis, and cardiogenic asthma. In this study, we developed a rapid and sensitive method to determine AMP concentration in mouse serum. A sensitive monoclonal antibody against AMP was produced by immunizing BALB/c mice with a well-characterized AMP2-keyhole limpet hemocyanin and coating antigen (hapten-AMP1-OVA). The coating antigen and goat anti-mouse IgG antibody were sprayed onto a nitrocellulose membrane representing the test and control lines, respectively. Under optimized conditions, the cut-off limits of the test strip were 50 ng/mL in 0.01 M phosphate buffered saline (pH 7.4) and 250 ng/mL in serum. The results were obtained within 10 min and revealed that this immunochromatographic strip test is a sensitive, rapid, and simple tool for the detection of AMP in serum.

中文翻译：

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使用免疫层析试纸检测血清中的氨茶碱

摘要 氨茶碱 (AMP) 是一种支气管扩张药，是茶碱（药理活性成分）的改良形式。AMP 通常用于治疗呼吸系统疾病，包括支气管哮喘、阻塞性肺气肿、气喘性支气管炎和心源性哮喘。在本研究中，我们开发了一种快速灵敏的方法来测定小鼠血清中的 AMP 浓度。一种针对 AMP 的敏感单克隆抗体是通过用充分表征的 AMP2-钥孔血蓝蛋白和涂层抗原 (半抗原-AMP1-OVA) 对 BALB/c 小鼠进行免疫接种来产生的。将包被抗原和山羊抗小鼠 IgG 抗体分别喷洒到代表测试线和对照线的硝酸纤维素膜上。在优化条件下，测试条的临界值在 0.01 M 磷酸盐缓冲盐水 (pH 7.4) 中为 50 ng/mL，在血清中为 250 ng/mL。